Evidence map›Paper›PMID 25774263›Full record

ArticleInternational journal of hematology-oncology and stem cell research2014

Differentiation of bone marrow mesenchymal stem cells into chondrocytes after short term culture in alkaline medium.

Farshad Homayouni Moghadam, Tahereh Tayebi, Maryam Dehghan, Gilda Eslami, Hamid Nadri, Alireza Moradi, Hassanali Vahedian-Ardakani, Kazem Barzegar

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of hematology-oncology and stem cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 22 citations in OpenAlex.

  1. Trial
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  5. Review
  6. Lamin A/C Is Dispensable to Mechanical Repression of Adipogenesis.International journal of molecular sciences · 2021
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  11. 2,3,7,8-Tetrachlorodibenzo-p-dioxin andDose-response : a publication of International Hormesis Society
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Farshad Homayouni MoghadamDepartment of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ; Neurobiomedical Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Tahereh TayebiDepartment of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Maryam DehghanNeurobiomedical Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Gilda EslamiDepartment of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Hamid NadriDepartment of Medicinal Chemistry, School of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Alireza MoradiDepartment of Medicinal Chemistry, School of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Hassanali Vahedian-ArdakaniDepartment of Internal Medicine, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Kazem BarzegarEnglish Language Department, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Shahid Sadoughi University of Medical Sciences and Health Services · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body. MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical factors such as pH could recommend new approaches for preparation of suitable scaffolds for bone and cartilage tissue engineering. In present study, the effect of alkaline medium on chondrogenic and osteogenic differentiation of rat MSCs was evaluated.

methodsMSCs were harvested from bone marrow of animals and then the response of passage1 and 2 of MSCs (P1 MSCs & P2 MSCs) to the culture in alkaline medium (pH: 8) was evaluated. Cytochemical and immunocytochemical staining were performed to distinguish chondrocytes and osteocytes. Real-time PCR was performed to evaluate the type II collagen and osteopontin mRNA levels.

resultsStaining for type II collagen, a chondrocytic specific marker, revealed that after one-week culture in alkaline medium, a considerable amount of P1 MSCs had shown chondrocytic morphology. By prolonging the culture period up to 4 weeks, osteogenic cells with expanded matrix and mineralized areas around them were appeared. Results of real-time PCR showed that P1 MSCs after one week culture in alkaline medium expressed highest rate of type II collagen and osteopontin mRNA among all groups.

conclusionThis study demonstrated that alkaline medium is a potent chondrogenic differentiation inducer for MSCs in their first passage.

Indexed as

Alkaline mediumChondrocyteMesenchymal stem cellOsteoblast

Identifiers

PMID25774263
PMCPMC4345293
OpenAlexW2992455840

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.