ArticleEnvironmental science and pollution research international2015
Assessment of Toxoplasma gondii levels in zebra mussel (Dreissena polymorpha) by real-time PCR: an organotropism study.
Article in Environmental science and pollution research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- A global synthesis of ecosystem services provided and disrupted by freshwater bivalve molluscs.Biological reviews of the Cambridge Philosophical Society · 2022Pooled it
- Comparison of two sentinel speciesMicrobiology spectrum · 2026Article
- Microplastics Facilitate Protozoan Pathogen Contamination in Shellfish.Microorganisms · 2026Article
- Clearance and persistence of Escherichia coli in the freshwater mussel Unio mancus.Scientific reports · 2022Article
- Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks withMicroorganisms · 2022Review
- Molecular Methods for the Detection ofMicroorganisms · 2021Review
- Comparison of PCR assays to detect Toxoplasma gondii oocysts in green-lipped mussels (Perna canaliculus).Parasitology research · 2019Article
- Real-time PCR detection of Toxoplasma gondii in surface water samples in São Paulo, Brazil.Parasitology research · 2019Article
- Impact of environmental factors on the emergence, transmission and distribution of Toxoplasma gondii.Parasites & vectors · 2016Review
- Environmental microbiology reveals the Earth secret life.Environmental science and pollution research international · 2015Article
- Environmental microbiology as a mosaic of explored ecosystems and issues.Environmental science and pollution research international · 2015Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Water quality is a public health concern that calls for relevant biomonitoring programs. Molecular tools such as polymerase chain reaction (PCR) are progressively becoming more sensitive and more specific than conventional techniques to detect pathogens in environmental samples such as water and organisms. The zebra mussel (Dreissena polymorpha) has already been demonstrated to accumulate and concentrate various human waterborne pathogens. In this study, first, a spiking experiment to evaluate detection levels of Toxoplasma gondii DNA in zebra mussel organs using real-time PCR was conducted. Overall, lower DNA levels in the hemolymph, digestive gland, and remaining tissues (gonad and foot) were detected compared to mantle, muscle, and gills. Second, an in vivo experiment with 1000 T. gondii oocysts per mussel and per day for 21 consecutive days, followed by 14 days of depuration time in protozoa-free water was performed. T. gondii DNA was detected in all organs, but greatest concentrations were observed in hemolymph and mantle tissues compared to the others organs at the end of the depuration period. These results suggest that (i) the zebra mussel is a potential new tool for measuring T. gondii concentrations and (ii) real-time PCR is a suitable method for pathogen detection in complex matrices such as tissues.
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