ArticleCurrent biology : CB2015
A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast.
Article in Current biology : CB, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Vps1 drives membrane constriction and fission necessary for endosomal protein sorting.The EMBO journal · 2026Article
- Dynamin2 functions as an accessory protein to reduce the rate of caveola internalization.The Journal of cell biology · 2023Article
- Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis.Journal of fungi (Basel, Switzerland) · 2022Article
- Article
- Phosphorylation of the WH2 domain in yeast Las17/WASP regulates G-actin binding and protein function during endocytosis.Scientific reports · 2021Article
- Emerging Mechanisms of Endocytosis inLife (Basel, Switzerland) · 2021Review
- Review
- Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein.Nature cell biology · 2020Article
- Physical Constraints and Forces Involved in Phagocytosis.Frontiers in immunology · 2020Review
- Yeast-model-based study identified myosin- and calcium-dependent calmodulin signalling as a potential target for drug intervention in chorea-acanthocytosis.Disease models & mechanisms · 2019Article
- Article
- Actin dynamics at focal adhesions: a common endpoint and putative therapeutic target for proteinuric kidney diseases.Kidney international · 2018Review
- Mechanisms of clathrin-mediated endocytosis.Nature reviews. Molecular cell biology · 2018Review
- A Genetic Screen for Investigating the Human Lysosomal CystineTransporter, Cystinosin.Scientific reports · 2018Article
- Mechanisms and modulation of microvesicle uptake in a model of alveolar cell communication.The Journal of biological chemistry · 2017Article
- Dynamin Autonomously Regulates Podocyte Focal Adhesion Maturation.Journal of the American Society of Nephrology : JASN · 2017Article
- Distinct complexes of yeast Snx4 family SNX-BARs mediate retrograde trafficking of Snc1 and Atg27.Traffic (Copenhagen, Denmark) · 2017Article
- Actin filaments as dynamic reservoirs for Drp1 recruitment.Molecular biology of the cell · 2016Article
- Clathrin-mediated endocytosis in budding yeast at a glance.Journal of cell science · 2016Review
- Insights into dynamin-associated disorders through analysis of equivalent mutations in the yeast dynamin Vps1.Microbial cell (Graz, Austria) · 2016Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Actin is critical for endocytosis in yeast cells, and also in mammalian cells under tension. However, questions remain as to how force generated through actin polymerization is transmitted to the plasma membrane to drive invagination and scission. Here, we reveal that the yeast dynamin Vps1 binds and bundles filamentous actin. Mutational analysis of Vps1 in a helix of the stalk domain identifies a mutant RR457-458EE that binds actin more weakly. In vivo analysis of Vps1 function demonstrates that the mutation disrupts endocytosis but not other functions of Vps1 such as vacuolar trafficking or peroxisome fission. The mutant Vps1 is stably expressed in cells and co-localizes with the endocytic reporters Abp1 and the amphiphysin Rvs167. Detailed analysis of individual endocytic patch behavior indicates that the mutation causes aberrant movements in later stages of endocytosis, consistent with a scission defect. Ultrastructural analysis of yeast cells using electron microscopy reveals a significant increase in invagination depth, further supporting a role for the Vps1-actin interaction during scission. In vitro analysis of the mutant protein demonstrates that--like wild-type Vps1--it is able to form oligomeric rings, but, critically, it has lost its ability to bundle actin filaments into higher-order structures. A model is proposed in which actin filaments bind Vps1 during invagination, and this interaction is important to transduce the force of actin polymerization to the membrane to drive successful scission.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.