ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2015
RZZ and Mad1 dynamics in Drosophila mitosis.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- CENcyclopedia: dynamic landscape of kinetochore architecture throughout the cell cycle.Nature communications · 2025Article
- ZW10: an emerging orchestrator of organelle dynamics during the cell division cycle.Journal of molecular cell biology · 2024Review
- A dynamic population of prophase CENP-C is required for meiotic chromosome segregation.PLoS genetics · 2023Article
- Kinetochore-catalyzed MCC formation: A structural perspective.IUBMB life · 2023Article
- The Disordered Spindly C-terminus Interacts with RZZ Subunits ROD-1 and ZWL-1 in the Kinetochore through the Same Sites in C. Elegans.Journal of molecular biology · 2021Article
- Polo regulates Spindly to prevent premature stabilization of kinetochore-microtubule attachments.The EMBO journal · 2020Article
- ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.Nucleic acids research · 2019Article
- The checkpoint protein Zw10 connects CAL1-dependent CENP-A centromeric loading and mitosis duration in Drosophila cells.PLoS genetics · 2019Article
- Recent Progress on the Localization of the Spindle Assembly Checkpoint Machinery to Kinetochores.Cells · 2019Review
- Epistatic Analysis of the Contribution of Rabs and Kifs to CATCHR Family Dependent Golgi Organization.Frontiers in cell and developmental biology · 2019Article
- Article
- Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis.Nature cell biology · 2018Article
- MAD1: Kinetochore Receptors and Catalytic Mechanisms.Frontiers in cell and developmental biology · 2018Review
- Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability.Open biology · 2016Article
- Downregulation of kinetochore-associated 1 gene increases lagging chromosomes and contributes to chromosomal instability in gastric cancer cells.Medicine internationalArticle
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
The presence or absence of Mad1 at kinetochores is a major determinant of spindle assembly checkpoint (SAC) activity, the surveillance mechanism that delays anaphase onset if one or more kinetochores remain unattached to spindle fibers. Among the factors regulating the levels of Mad1 at kinetochores is the Rod, Zw10, and Zwilch (RZZ) complex, which is required for Mad1 recruitment through a mechanism that remains unknown. The relative dynamics and interactions of Mad1 and RZZ at kinetochores have not been extensively investigated, although Mad1 has been reported to be stably recruited to unattached kinetochores. In this study, we directly compare Mad1-green fluorescent protein (GFP) turnover dynamics on unattached Drosophila kinetochores with that of RZZ, tagged either with GFP-Rod or GFP-Zw10. We find that nearly 40 % of kinetochore-bound Mad1 has a significant dynamic component, turning over with a half-life of 12 s. RZZ in contrast is essentially stable on unattached kinetochores. In addition, we report that a fraction of RZZ and Mad1 can co-immunoprecipitate, indicating that the genetically determined recruitment hierarchy (in which Mad1 depends on RZZ) may reflect a physical association of the two complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.