ArticleFree radical biology & medicine2015
Reinterpreting the best biomarker of oxidative stress: The 8-iso-PGF(2α)/PGF(2α) ratio distinguishes chemical from enzymatic lipid peroxidation.
Article in Free radical biology & medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 4 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
75 citing papers in PubMed, 4 syntheses or guidelines pooled it, 120 citations in OpenAlex.
- Urinary oxidative stress biomarkers are associated with preterm birth: an Environmental Influences on Child Health Outcomes program study.American journal of obstetrics and gynecology · 2023Pooled it
- Circulating biomarkers of antioxidant status and oxidative stress in people with cystic fibrosis: A systematic review and meta-analysis.Redox biology · 2020Pooled it
- Strategies to decrease oxidative stress biomarker levels in human medical conditions: A meta-analysis on 8-iso-prostaglandin FRedox biology · 2018Pooled it
- Classifying oxidative stress by FRedox biology · 2017Pooled it
- Serum 8-isoprostane levels in patients with resistant oral lichen planus before and after treatment with lycopene: a randomized clinical trial.BMC oral health · 2021Trial
- Effect of long-term vitamin E and selenium supplementation on urine F2-isoprostanes, a biomarker of oxidative stress.Free radical biology & medicine · 2016Trial
- Eicosanoids as Biomarkers of Hyperfiltration-Mediated Injury in CKD.Kidney international reports · 2026Article
- A liquid chromatography- tandem mass spectrometry method for the analysis of oxidative stress, nitrative stress, metabolic, and inflammatory biomarkers in serum: comparison to urinary concentrations.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2026Article
- 8-Iso-Prostaglandin F2α and Lipoxygenase Gene Expression as Candidate Molecular Markers of Training Adaptation in Professional Volleyball Players: A Pilot Study.International journal of molecular sciences · 2026Article
- Development of a Biology-Informed Chemical Mixture Index for Oxidative Stress and Mortality in NHANES 2005-2010: A Survey-Weighted Quantile G-Computation Approach.medRxiv : the preprint server for health sciences · 2026Article
- Elevated Amniotic Fluid 8-Iso-Prostaglandin FAntioxidants (Basel, Switzerland) · 2026Article
- Next-Generation Antioxidants in Cardiovascular Disease: Mechanistic Insights and Emerging Therapeutic Strategies.Antioxidants (Basel, Switzerland) · 2026Review
- Isoprostanes as biomarkers of oxidative stress across human diseases and skin pathologies.Frontiers in molecular biosciences · 2026Review
- Camphorquinone-induced phospholipase AJournal of dental sciences · 2026Article
- Research Progress of Ferroptosis in Cerebral Infarction.Brain and behavior · 2026Review
- Clinical Evaluation of Oxidative Stress Biomarkers in Cirrhosis: Associations with Child-Pugh Class and Hepatic Encephalopathy.Diagnostics (Basel, Switzerland) · 2025Article
- Role of nitric oxide, prostaglandins, thromboxanes and endothelins in lung cancer: An overview.World journal of clinical cases · 2025Review
- Effects of Methotrexate and Tofacitinib on Mitochondrial Function and Oxidative Stress in Human Synovial Cells In Vitro.International journal of molecular sciences · 2025Article
- Association between organic micropollutants in tap water and human exposure and birth outcomes: Implications for environmental health in northern Puerto Rico.Journal of hazardous materials · 2025Article
- Unveiling the Hidden Threat: Per- and Polyfluoroalkyl Substance Pollution's Distinct Traits and Crucial Impacts on Lung Function in Chinese Plateau Populations.Environment & health (Washington, D.C.) · 2025Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The biomarker 8-iso-prostaglandin F2α (8-iso-PGF2α) is regarded as the gold standard for detection of excessive chemical lipid peroxidation in humans. However, biosynthesis of 8-iso-PGF2α via enzymatic lipid peroxidation by prostaglandin-endoperoxide synthases (PGHSs), which are significantly induced in inflammation, could lead to incorrect biomarker interpretation. To resolve the ambiguity with this biomarker, the ratio of 8-iso-PGF2α to prostaglandin F2α (PGF2α) is established as a quantitative measure to distinguish enzymatic from chemical lipid peroxidation in vitro, in animal models, and in humans. Using this method, we find that chemical lipid peroxidation contributes only 3% to the total 8-iso-PGF2α in the plasma of rats. In contrast, the 8-iso-PGF2α levels in plasma of human males are generated >99% by chemical lipid peroxidation. This establishes the potential for an alternate pathway of biomarker synthesis, and draws into question the source of increases in 8-iso-PGF2α seen in many human diseases. In conclusion, increases in 8-iso-PGF2α do not necessarily reflect increases in oxidative stress; therefore, past studies using 8-iso-PGF2α as a marker of oxidative stress may have been misinterpreted. The 8-iso-PGF2α/PGF2α ratio can be used to distinguish biomarker synthesis pathways and thus confirm the potential change in oxidative stress in the myriad of disease and chemical exposures known to induce 8-iso-PGF2α.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.