ArticleBiomaterials2015
Capture of endothelial cells under flow using immobilized vascular endothelial growth factor.
Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- Supramolecular engineering of fibrinogen into a fibrin matrix with enhanced bioactive properties.Bioactive materials · 2027Article
- Biodegradable Synthetic Graft with Sustained Hydrogen Sulfide Release Promotes Endothelial Cell Growth.Macromolecular bioscience · 2025Article
- Immune-mediated regeneration of cell-free vascular grafts in an ovine model.NPJ Regenerative medicine · 2025Article
- Harnessing the potential of monocytes/macrophages to regenerate tissue-engineered vascular grafts.Cardiovascular research · 2024Review
- Monocyte Recruitment for Vascular Tissue Regeneration.Advanced healthcare materials · 2022Article
- Bio-inspired hemocompatible surface modifications for biomedical applications.Progress in materials science · 2022Article
- An efficient strategy to recellularization of a rat aorta scaffold: an optimized decellularization, detergent removal, and Apelin-13 immobilization.Biomaterials research · 2022Article
- Generating Monocyte-Derived Endothelial-like Cells for Vascular Regeneration.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Next-generation tissue-engineered heart valves with repair, remodelling and regeneration capacity.Nature reviews. Cardiology · 2021Review
- Cell-free vascular grafts that grow with the host.Advanced functional materials · 2020Article
- Surface modification of small intestine submucosa in tissue engineering.Regenerative biomaterials · 2020Review
- Endothelialization of arterial vascular grafts by circulating monocytes.Nature communications · 2020Article
- Hierarchical Capillary Coating to Biofunctionlize Drug-Eluting Stent for Improving Endothelium Regeneration.Research (Washington, D.C.) · 2020Article
- Sphingosine-1-phosphate in Endothelial Cell Recellularization Improves Patency and Endothelialization of Decellularized Vascular Grafts In Vivo.International journal of molecular sciences · 2019Article
- Implantation of VEGF-functionalized cell-free vascular grafts: regenerative and immunological response.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Identification of active signaling pathways by integrating gene expression and protein interaction data.BMC systems biology · 2018Article
- Tissue Engineering at the Blood-Contacting Surface: A Review of Challenges and Strategies in Vascular Graft Development.Advanced healthcare materials · 2018Review
- In Vivo Performance of Decellularized Vascular Grafts: A Review Article.International journal of molecular sciences · 2018Review
- Improving in vivo outcomes of decellularized vascular grafts via incorporation of a novel extracellular matrix.Biomaterials · 2017Article
- Translational Challenges in Cardiovascular Tissue Engineering.Journal of cardiovascular translational research · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We demonstrate the ability of immobilized vascular endothelial growth factor (VEGF) to capture endothelial cells (EC) with high specificity under fluid flow. To this end, we engineered a surface consisting of heparin bound to poly-l-lysine to permit immobilization of VEGF through the C-terminal heparin-binding domain. The immobilized growth factor retained its biological activity as shown by proliferation of EC and prolonged activation of KDR signaling. Using a microfluidic device we assessed the ability to capture EC under a range of shear stresses from low (0.5 dyne/cm(2)) to physiological (15 dyne/cm(2)). Capture was significant for all shear stresses tested. Immobilized VEGF was highly selective for EC as evidenced by significant capture of human umbilical vein and ovine pulmonary artery EC but no capture of human dermal fibroblasts, human hair follicle derived mesenchymal stem cells, or mouse fibroblasts. Further, VEGF could capture EC from mixtures with non-EC under low and high shear conditions as well as from complex fluids like whole human blood under high shear. Our findings may have far reaching implications, as they suggest that VEGF could be used to promote endothelialization of vascular grafts or neovascularization of implanted tissues by rare but continuously circulating EC.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.