Evidence map›Paper›PMID 25770999›Full record

ArticleBiomaterials2015

Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces.

Rene Olivares-Navarrete, Sandra E Rodil, Sharon L Hyzy, Ginger R Dunn, Argelia Almaguer-Flores, Zvi Schwartz, Barbara D Boyan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
4.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 105 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Rene Olivares-NavarreteDepartment of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA, USA.
Sandra E RodilInstituto de Investigaciones en Materiales, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico.
Sharon L HyzyDepartment of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA, USA.
Ginger R DunnWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Argelia Almaguer-FloresFacultad de Odontologia, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico.
Zvi SchwartzDepartment of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA, USA.
Barbara D BoyanDepartment of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA. Electronic address: bboyan@vcu.edu.
Virginia Commonwealth University · USGeorgia Institute of Technology · USUniversidad Nacional Autónoma de México · MX

Funding

Mechanisms of Cell/Surface InteractionR01AR052102 · NIAMS · VIRGINIA COMMONWEALTH UNIVERSITY · PI BOYAN, BARBARA D. · 2006 to 2015
$3.3M
NIAMS NIH HHS AR052102NIAMS NIH HHS R01 AR052102
6 · The paper itself

Abstract

Surface roughness, topography, chemistry, and energy promote osteoblast differentiation and increase osteogenic local factor production in vitro and bone-to-implant contact in vivo, but the mechanisms involved are not well understood. Knockdown of integrin heterodimer alpha2beta1 (α2β1) blocks the osteogenic effects of the surface, suggesting signaling by this integrin homodimer is required. The purpose of the present study was to separate effects of surface chemistry and surface structure on integrin expression by coating smooth or rough titanium (Ti) substrates with graphitic carbon, retaining surface morphology but altering surface chemistry. Ti surfaces (smooth [Ra < 0.4 μm], rough [Ra ≥ 3.4 μm]) were sputter-coated using a magnetron sputtering system with an ultrapure graphite target, producing a graphitic carbon thin film. Human mesenchymal stem cells and MG63 osteoblast-like cells had higher mRNA for integrin subunits α1, α2, αv, and β1 on rough surfaces in comparison to smooth, and integrin αv on graphitic-carbon-coated rough surfaces in comparison to Ti. Osteogenic differentiation was greater on rough surfaces in comparison to smooth, regardless of chemistry. Silencing integrins β1, α1, or α2 decreased osteoblast maturation on rough surfaces independent of surface chemistry. Silencing integrin αv decreased maturation only on graphitic carbon-coated surfaces, not on Ti. These results suggest a major role of the integrin β1 subunit in roughness recognition, and that integrin alpha subunits play a major role in surface chemistry recognition.

Indexed as

Cell AdhesionCell DifferentiationCell LineCoated Materials, BiocompatibleGene SilencingGraphiteHumansIntegrinsMesenchymal Stem CellsOsteoblastsProtein SubunitsSurface PropertiesTitaniumCoated Materials, BiocompatibleGraphiteIntegrinsProtein SubunitsTitaniumGraphitic carbon coatingGrowth factorsMesenchymal stem cellsOsteoblast differentiationTitanium

Identifiers

PMID25770999
PMCPMC4636027
OpenAlexW2082957960

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.