ArticleThe Plant cell2015
Determining the subcellular location of synthesis and assembly of the cell wall polysaccharide (1,3; 1,4)-β-D-glucan in grasses.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 69 citations in OpenAlex.
- The Plant Cell Wall: From Structural Diversity and Biosynthesis to Integrity Signaling and Physiological Functions.Plants (Basel, Switzerland) · 2026Review
- CESA7 and microtubules pattern complex secondary cell walls in explosive fruit of Cardamine hirsuta.The Plant cell · 2026Article
- β-Glucan Synthesis Gene Family in Crops: Advances in Classification, Function, and Regulation.Current issues in molecular biology · 2025Review
- The knockout of ClaCSLH1 induced dwarfing in watermelon.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Colocalising proteins and polysaccharides in plants for cell wall and trafficking studies.Frontiers in plant science · 2024Article
- Tip Growth Defective1 interacts with the cellulose synthase complex to regulate cellulose synthesis in Arabidopsis thaliana.PloS one · 2024Article
- Enzymes in 3D: Synthesis, remodelling, and hydrolysis of cell wall (1,3;1,4)-β-glucans.Plant physiology · 2023Article
- Galls induced by a root-knot nematode in Petroselinum crispum (Mill.): impacts on host development, histology, and cell wall dynamics.Protoplasma · 2023Article
- Unraveling the Potential of β-D-Glucans inLife (Basel, Switzerland) · 2023Review
- Study of Dynamic Accumulation in β-D-Glucan in Oat (Polymers · 2022Article
- Identification of candidate MYB transcription factors that influenceFrontiers in plant science · 2022Article
- Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries.Biotechnology for biofuels · 2021Review
- A Pipeline towards the Biochemical Characterization of theInternational journal of molecular sciences · 2021Article
- Article
- Biochemical and Functional Characterization of GALT8, anFrontiers in plant science · 2021Article
- Seed coat development in explosively dispersed seeds of Cardamine hirsuta.Annals of botany · 2020Article
- Integrative Multi-omics Analyses of Barley Rootzones under Salinity Stress Reveal Two Distinctive Salt Tolerance Mechanisms.Plant communications · 2020Article
- Non-Starch Polysaccharides in Durum Wheat: A Review.International journal of molecular sciences · 2020Review
- Overexpression of HvCslF6 in barley grain alters carbohydrate partitioning plus transfer tissue and endosperm development.Journal of experimental botany · 2020Article
- Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research.Biodesign research · 2020Review
Corrections and comments
- Commented on by
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current dogma for cell wall polysaccharide biosynthesis is that cellulose (and callose) is synthesized at the plasma membrane (PM), whereas matrix phase polysaccharides are assembled in the Golgi apparatus. We provide evidence that (1,3;1,4)-β-D-glucan (mixed-linkage glucan [MLG]) does not conform to this paradigm. We show in various grass (Poaceae) species that MLG-specific antibody labeling is present in the wall but absent over Golgi, suggesting it is assembled at the PM. Antibodies to the MLG synthases, cellulose synthase-like F6 (CSLF6) and CSLH1, located CSLF6 to the endoplasmic reticulum, Golgi, secretory vesicles, and the PM and CSLH1 to the same locations apart from the PM. This pattern was recreated upon expression of VENUS-tagged barley (Hordeum vulgare) CSLF6 and CSLH1 in Nicotiana benthamiana leaves and, consistent with our biochemical analyses of native grass tissues, shown to be catalytically active with CSLF6 and CSLH1 in PM-enriched and PM-depleted membrane fractions, respectively. These data support a PM location for the synthesis of MLG by CSLF6, the predominant enzymatically active isoform. A model is proposed to guide future experimental approaches to dissect the molecular mechanism(s) of MLG assembly.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.