ArticleInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases2015
Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals.
Article in Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The trial behind it
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- Completion of the Genome Sequence of a Historic CDV Vaccine Strain, Rockborn: Evolutionary and Epidemiologic Implications.Veterinary sciences · 2026Article
- Development of Multiplex PCR assay for detection of Canine Infectious Respiratory Disease Complex (CIRDC) pathogens in dogs.Frontiers in cellular and infection microbiology · 2025Article
- Article
- Canine morbillivirus (CDV): a review on current status, emergence and the diagnostics.Virusdisease · 2022Review
- Development of recombinase polymerase amplification assays for rapid and visual detection of canine distemper virus infecting giant panda.BMC veterinary research · 2021Article
- One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus.The Journal of veterinary medical science · 2019Article
- Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection ofYonago acta medica · 2016Article
- Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification (LAMP) based on internal transcribed spacer 2 (ITS2) sequence.Scientific reports · 2016Article
- An Innovative Method for Rapid Identification and Detection of Vibrio alginolyticus in Different Infection Models.Frontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although widespread vaccination against canine distemper virus (CDV) has been conducted for many decades, several canine distemper outbreaks in vaccinated animals have been reported frequently. In order to detect and differentiate the wild-type and vaccine strains of the CDV from the vaccinated animals, a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed. A set of four primers-two internal and two external-were designed to target the H gene for the specific detection of wild-type CDV variants. The CDV-H RT-LAMP assay rapidly amplified the target gene, within 60 min, using a water bath held at a constant temperature of 65°C. The assay was 100-fold more sensitive than conventional RT-PCR, with a detection limit of 10(-1)TCID50ml(-1). The system showed a preference for wild-type CDV, and exhibited less sensitivity to canine parvovirus, canine adenovirus type 1 and type 2, canine coronavirus, and canine parainfluenza virus. The assay was validated using 102 clinical samples obtained from vaccinated dog farms, and the results were comparable to a multiplex nested RT-PCR assay. The specific CDV-H RT-LAMP assay provides a simple, rapid, and sensitive tool for the detection of canines infected with wild-type CDV from canines vaccinated with attenuated vaccine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.