Evidence map›Paper›PMID 25769653›Full record

ArticleStem cells translational medicine2015

Development of a universal RNA beacon for exogenous gene detection.

Yuanjian Guo, Zhongju Lu, Ira Stephen Cohen, Suzanne Scarlata

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Yuanjian GuoDepartment of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA.
Zhongju LuDepartment of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA.
Ira Stephen CohenDepartment of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA.
Suzanne ScarlataDepartment of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA Suzanne.Scarlata@stonybrook.edu.
Stony Brook University · US

Funding

Spatial Models of Cell Regulatory NetworksR01GM072853 · NIGMS · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI HONE, JAMES C, IYENGAR, SRINIVAS RAVI V · 2005 to 2019
$6.1M
ACTIVATION OF PHOSPHOLIPASE C BETA BY G PROTEINSR01GM053132 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI SCARLATA, SUZANNE F · 1995 to 2014
$3.5M
From human keratinocytes to biological pacemakersR01HL111401 · NHLBI · STATE UNIVERSITY NEW YORK STONY BROOK · PI COHEN, IRA S · 2012 to 2015
$2.6M
NHLBI NIH HHS HL-111401NHLBI NIH HHS R01 HL111401NIGMS NIH HHS GM053132NIGMS NIH HHS R01 GM053132NIGMS NIH HHS R01 GM072853
6 · The paper itself

Abstract

Stem cell therapy requires a nontoxic and high-throughput method to achieve a pure cell population to prevent teratomas that can occur if even one cell in the implant has not been transformed. A promising method to detect and separate cells expressing a particular gene is RNA beacon technology. However, developing a successful, specific beacon to a particular transfected gene can take months to develop and in some cases is impossible. Here, we report on an off-the-shelf universal beacon that decreases the time and cost of applying beacon technology to select any living cell population transfected with an exogenous gene.

Indexed as

AnimalsCell TrackingDogsFluorescent DyesHumansHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMesenchymal Stem CellsMolecular ProbesPotassium ChannelsRNA, MessengerFluorescent DyesHCN2 protein, humanHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMolecular ProbesPotassium ChannelsRNA, MessengerFluorescence-activated cell sorting analysisGene expressionRNA beaconsStem cell selectionStem cell separation

Identifiers

PMID25769653
PMCPMC4414216
OpenAlexW2062122098

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.