ArticleACS nano2015
Nanopore detection of 8-oxoguanine in the human telomere repeat sequence.
Article in ACS nano, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 96 citations in OpenAlex.
- Nanopore Studies of DNA Damage and G-quadruplex Folding in the Human Telomere Sequence.Chimia · 2026Article
- G-Quadruplex Unwinding Molecular Mechanisms by Helicases and Their Applications.International journal of molecular sciences · 2026Review
- Single-Molecule Biodosimetry.Analytical chemistry · 2025Article
- A new compression strategy to reduce the size of nanopore sequencing data.Genome research · 2025Article
- Impact of a single 8-oxoguanine lesion on Watson-Crick and Hoogsteen base pair hybridization in telomeric DNA quantified using single-molecule force spectroscopy.Nucleic acids research · 2025Article
- The enduring advantages of the SLOW5 file format for raw nanopore sequencing data.GigaScience · 2025Article
- Nanopore- and AI-empowered microbial viability inference.GigaScience · 2025Article
- 8-OxodG: A Potential Biomarker for Chronic Oxidative Stress Induced by High-LET Radiation.DNA · 2024Article
- Interactive visualization of nanopore sequencing signal data with Squigualiser.Bioinformatics (Oxford, England) · 2024Article
- Nanopore Direct RNA Sequencing for Modified Uridine Nucleotides Yields Signals Dependent on the Physical Properties of the Modified Base.Israel journal of chemistry · 2024Article
- Long-read sequencing for brain tumors.Frontiers in oncology · 2024Review
- Advantages and challenges associated with bisulfite-assisted nanopore direct RNA sequencing for modifications.RSC chemical biology · 2023Article
- Aldehyde-Associated Mutagenesis─Current State of Knowledge.Chemical research in toxicology · 2023Review
- Structure and Dynamics of dsDNA in Cell-like Environments.Entropy (Basel, Switzerland) · 2022Review
- Purification of mammalian telomeric DNA for single-molecule analysis.Nature protocols · 2022Review
- Quantification of 8-International journal of molecular sciences · 2022Article
- De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide.Nature nanotechnology · 2022Article
- Biomarkers of nucleic acid oxidation - A summary state-of-the-art.Redox biology · 2021Article
- Detection and Discrimination of DNA Adducts Differing in Size, Regiochemistry, and Functional Group by Nanopore Sequencing.Chemical research in toxicology · 2020Article
- Long-read human genome sequencing and its applications.Nature reviews. Genetics · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The human telomere repeat sequence 5'-TTAGGG-3' is a hot spot for oxidation at guanine, yielding 8-oxo-7,8-dihydroguanine (OG), a biomarker of oxidative stress. Telomere shortening resulting from oxidation will ultimately induce cellular senescence. In this study, α-hemolysin (α-HL) nanopore technology was applied to detect and quantify OG in the human telomeric DNA sequence. This repeat sequence adopts a basket G-quadruplex in the NaCl electrolyte used for analysis that enters the α-HL channel, slowly unfolds, and translocates. The basket fold containing OG disrupts the structure, leading to >10× increase in the unfolding kinetics without yielding a detectable current pattern. Therefore, detection of OG with α-HL required labeling of OG with aminomethyl-[18-crown-6] using a mild oxidant. The labeled OG yielded a pulse-like signal in the current vs time trace when the DNA strand was electrophoretically passed through α-HL in NaCl electrolyte. However, the rate of translocation was too slow using NaCl salts, leading us to further refine the method. A mixture of NH4Cl and LiCl electrolytes induced the propeller fold that unravels quickly outside the α-HL channel. This electrolyte allowed observation of the labeled OG, while providing a faster recording of the currents. Lastly, OG distributions were probed with this method in a 120-mer stretch of the human telomere sequence exposed to the cellular oxidant (1)O2. Single-molecule profiles determined the OG distributions to be random in this context. Application of the method in nanomedicine can potentially address many questions surrounding oxidative stress and telomere attrition observed in various disease phenotypes including prostate cancer and diabetes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.