ArticleBMC genomics2015
The germline of the malaria mosquito produces abundant miRNAs, endo-siRNAs, piRNAs and 29-nt small RNAs.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- microRNA profiles and functions in mosquitoes.PLoS neglected tropical diseases · 2018Pooled it
- The genome and sex-dependent responses to temperature in the common yellow butterfly, Eurema hecabe.BMC biology · 2023Article
- microRNA Expression Dynamics inInsects · 2023Article
- miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta.Scientific reports · 2021Article
- A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons.Genome research · 2021Article
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- Review
- Global Analysis of Small Non-Coding RNA Populations across Tissues in the Malaria Vector,Insects · 2020Article
- Conserved Small Nucleotidic Elements at the Origin of Concerted piRNA Biogenesis from Genes and lncRNAs.Cells · 2020Article
- De novo profiling of RNA viruses in Anopheles malaria vector mosquitoes from forest ecological zones in Senegal and Cambodia.BMC genomics · 2019Article
- Small RNA-Seq Analysis Reveals miRNA Expression Dynamics Across Tissues in the Malaria Vector,G3 (Bethesda, Md.) · 2019Article
- MicroRNAs from saliva of anopheline mosquitoes mimic human endogenous miRNAs and may contribute to vector-host-pathogen interactions.Scientific reports · 2019Article
- Article
- Highly focused transcriptional response of Anopheles coluzzii to O'nyong nyong arbovirus during the primary midgut infection.BMC genomics · 2018Article
- Identification and verification of potential piRNAs from domesticated yak testis.Reproduction (Cambridge, England) · 2018Article
- MicroRNA Tissue Atlas of the Malaria MosquitoG3 (Bethesda, Md.) · 2018Article
- Characterization and comparative analysis of microRNAs in the rice pest Sogatella furcifera.PloS one · 2018Article
- Histone-derived piRNA biogenesis depends on the ping-pong partners Piwi5 and Ago3 in Aedes aegypti.Nucleic acids research · 2017Article
- Transposable Element Bm1645 is a Source of BmAGO2-associated Small RNAs that affect its expression in Bombyx mori.BMC genomics · 2017Article
- Comparative expression profile of microRNAs in Anopheles anthropophagus midgut after blood-feeding and Plasmodium infection.Parasites & vectors · 2017Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
backgroundSmall RNAs include different classes essential for endogenous gene regulation and cellular defence against genomic parasites. However, a comprehensive analysis of the small RNA pathways in the germline of the mosquito Anopheles gambiae has never been performed despite their potential relevance to reproductive capacity in this malaria vector.
resultsWe performed small RNA deep sequencing during larval and adult gonadogenesis and find that they predominantly express four classes of regulatory small RNAs. We identified 45 novel miRNA precursors some of which were sex-biased and gonad-enriched , nearly doubling the number of previously known miRNA loci. We also determine multiple genomic clusters of 24-30 nt Piwi-interacting RNAs (piRNAs) that map to transposable elements (TEs) and 3'UTR of protein coding genes. Unusually, many TEs and the 3'UTR of some endogenous genes produce an abundant peak of 29-nt small RNAs with piRNA-like characteristics. Moreover, both sense and antisense piRNAs from TEs in both Anopheles gambiae and Drosophila melanogaster reveal novel features of piRNA sequence bias. We also discovered endogenous small interfering RNAs (endo-siRNAs) that map to overlapping transcripts and TEs.
conclusionsThis is the first description of the germline miRNome in a mosquito species and should prove a valuable resource for understanding gene regulation that underlies gametogenesis and reproductive capacity. We also provide the first evidence of a piRNA pathway that is active against transposons in the germline and our findings suggest novel piRNA sequence bias. The contribution of small RNA pathways to germline TE regulation and genome defence in general is an important finding for approaches aimed at manipulating mosquito populations through the use of selfish genetic elements.
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