ArticleBMC genomics2015
Transcriptome analysis of Panax vietnamensis var. fuscidicus discovers putative ocotillol-type ginsenosides biosynthesis genes and genetic markers.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Leveraging next-generation sequencing technology for the study of ginsenosides biosynthesis and exploring DNA markers in the endemic species Panax vietnamensis.Molecular biology reports · 2024Pooled it
- Recombinant single-chain variable fragment-based immunoassay for the authentication of Vietnamese ginseng via quantitative detection of majonoside R2.Journal of ginseng research · 2026Article
- De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L.Scientific reports · 2025Article
- Transcriptomic and physiological analysis of atractylodes chinensis in response to drought stress reveals the putative genes related to sesquiterpenoid biosynthesis.BMC plant biology · 2024Article
- Suspension cell cultures ofFrontiers in plant science · 2024Article
- Comparative genomics reveals the diversification of triterpenoid biosynthesis and origin of ocotillol-type triterpenes in Panax.Plant communications · 2023Article
- Article
- Comparative transcriptome and metabolome analyses of fourJournal of ginseng research · 2023Article
- Transcriptome analysis and development of EST-SSR markers in Anoectochilus emeiensis (Orchidaceae).PloS one · 2022Article
- Article
- De novo whole-genome assembly and discovery of genes involved in triterpenoid saponin biosynthesis of Vietnamese ginseng (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Ginsenosides inActa pharmaceutica Sinica. B · 2021Review
- Metabolomes and transcriptomes revealed the saponin distribution in root tissues ofJournal of ginseng research · 2020Article
- De novo assembly and Transcriptome characterization of an endemic species of Vietnam, Panax vietnamensis Ha et Grushv., including the development of EST-SSR markers for population genetics.BMC plant biology · 2020Article
- Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.).Genetics and molecular biology · 2020Article
- Till 2018: a survey of biomolecular sequences in genusJournal of ginseng research · 2020Review
- Article
- Integrated Chemical and Transcriptomic Analysis Reveals the Distribution of Protopanaxadiol- and Protopanaxatriol-Type Saponins inMolecules (Basel, Switzerland) · 2018Article
- Identification and Characterization of Genes Involved in Benzylisoquinoline Alkaloid Biosynthesis inFrontiers in plant science · 2018Article
- Isoform Sequencing Provides a More Comprehensive View of the Panax ginseng Transcriptome.Genes · 2017Article
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9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundP. vietnamensis var. fuscidiscus, called "Yesanqi" in Chinese, is a new variety of P. vietnamensis, which was first found in Jinping County, the southern part of Yunnan Province, China. Compared with other Panax plants, this species contains higher content of ocotillol-type saponin, majonoside R2. Despite the pharmacological importance of ocotillol-type saponins, little is known about their biosynthesis in plants. Hence, P. vietnamensis var. fuscidiscus is a suitable medicinal herbal plant species to study biosynthesis of ocotillol-type saponins. In addition, the available genomic information of this important herbal plant is lacking.
resultsTo investigate the P. vietnamensis var. fuscidiscus transcriptome, Illumina HiSeq™ 2000 sequencing platform was employed. We produced 114,703,210 clean reads, assembled into 126,758 unigenes, with an average length of 1,304 bp and N50 of 2,108 bp. Among these 126,758 unigenes, 85,214 unigenes (67.23%) were annotated based on the information available from the public databases. The transcripts encoding the known enzymes involved in triterpenoid saponins biosynthesis were identified in our Illumina dataset. A full-length cDNA of three Squalene epoxidase (SE) genes were obtained using reverse transcription PCR (RT-PCR) and the expression patterns of ten unigenes were analyzed by reverse transcription quantitative real-time PCR (RT-qPCR). Furthermore, 15 candidate cytochrome P450 genes and 17 candidate UDP-glycosyltransferase genes most likely to involve in triterpenoid saponins biosynthesis pathway were discovered from transcriptome sequencing of P. vietnamensis var. fuscidiscus. We further analyzed the data and found 21,320 simple sequence repeats (SSRs), 30 primer pairs for SSRs were randomly selected for validation of the amplification and polymorphism in 13 P. vietnamensis var. fuscidiscus accessions. Meanwhile, five major triterpene saponins in roots of P. vietnamensis var. fuscidicus were determined using high performance liquid chromatography (HPLC) and evaporative light scattering detector (ELSD).
conclusionsThe genomic resources generated from P. vietnamensis var. fuscidiscus provide new insights into the identification of putative genes involved in triterpenoid saponins biosynthesis pathway. This will facilitate our understanding of the biosynthesis of triterpenoid saponins at molecular level. The SSR markers identified and developed in this study show genetic diversity for this important crop and will contribute to marker-assisted breeding for P. vietnamensis var. fuscidiscus.
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