Evidence map›Paper›PMID 25765328›Full record

ArticleBrain : a journal of neurology2015

Astrocyte uncoupling as a cause of human temporal lobe epilepsy.

Peter Bedner, Alexander Dupper, Kerstin Hüttmann, Julia Müller, Michel K Herde, Pavel Dublin, Tushar Deshpande, Johannes Schramm, Ute Häussler, Carola A Haas and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain : a journal of neurology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 176 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
176citing papers in PubMed, 1 pooled it
12.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

176 citing papers in PubMed, 1 synthesis or guideline pooled it, 317 citations in OpenAlex.

  1. Pooled it
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  7. Membrane voltage multistability in coupled glial cells.bioRxiv : the preprint server for biology · 2026
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116 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 2 countries.

Peter Bedner1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
Alexander Dupper1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
Kerstin Hüttmann1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
Julia Müller1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
Michel K Herde1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
Pavel Dublin1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany Current address: Institute of Neurobiology, University of Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany.
Tushar Deshpande1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
Johannes Schramm2 Department of Neurosurgery, Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
Ute Häussler3 Experimental Epilepsy Research, Department of Neurosurgery, University Hospital Freiburg, 79106 Freiburg, Germany.
Carola A Haas3 Experimental Epilepsy Research, Department of Neurosurgery, University Hospital Freiburg, 79106 Freiburg, Germany.
Christian Henneberger1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany 4 UCL Institute of Neurology, UCL, London WC1N 3BG, UK.
Martin Theis1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
Christian Steinhäuser1 Institute of Cellular Neurosciences and Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany Christian.Steinhaeuser@ukb.uni-bonn.de.
University of Bonn · DEUniversity Medical Center Freiburg · DEHeinrich Heine University Düsseldorf · DEUniversity College London · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glial cells are now recognized as active communication partners in the central nervous system, and this new perspective has rekindled the question of their role in pathology. In the present study we analysed functional properties of astrocytes in hippocampal specimens from patients with mesial temporal lobe epilepsy without (n = 44) and with sclerosis (n = 75) combining patch clamp recording, K(+) concentration analysis, electroencephalography/video-monitoring, and fate mapping analysis. We found that the hippocampus of patients with mesial temporal lobe epilepsy with sclerosis is completely devoid of bona fide astrocytes and gap junction coupling, whereas coupled astrocytes were abundantly present in non-sclerotic specimens. To decide whether these glial changes represent cause or effect of mesial temporal lobe epilepsy with sclerosis, we developed a mouse model that reproduced key features of human mesial temporal lobe epilepsy with sclerosis. In this model, uncoupling impaired K(+) buffering and temporally preceded apoptotic neuronal death and the generation of spontaneous seizures. Uncoupling was induced through intraperitoneal injection of lipopolysaccharide, prevented in Toll-like receptor4 knockout mice and reproduced in situ through acute cytokine or lipopolysaccharide incubation. Fate mapping confirmed that in the course of mesial temporal lobe epilepsy with sclerosis, astrocytes acquire an atypical functional phenotype and lose coupling. These data suggest that astrocyte dysfunction might be a prime cause of mesial temporal lobe epilepsy with sclerosis and identify novel targets for anti-epileptogenic therapeutic intervention.

Indexed as

AnimalsAstrocytesElectroencephalographyEpilepsy, Temporal LobeGap JunctionsHippocampusHumansMaleMiceSclerosisSeizuresgap junction couplinggap junction protein alpha 1hippocampal sclerosisinflammationtemporal lobe epilepsy

Identifiers

PMID25765328
PMCPMC5963418
OpenAlexW2147191089

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.