ArticleBMC genomics2015
Comparative analysis among the small RNA populations of source, sink and conductive tissues in two different plant-virus pathosystems.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Dynamics of small RNAs in a red-fruited wine grape cultivar infected with Grapevine red blotch virus.BMC genomics · 2025Article
- Plant YTHDF proteins are direct effectors of antiviral immunity against an N6-methyladenosine-containing RNA virus.The EMBO journal · 2023Article
- Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (Viruses · 2023Article
- Parasitic plant small RNA analyses unveil parasite-specific signatures of microRNA retention, loss, and gain.Plant physiology · 2022Article
- Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis.Genome biology · 2021Article
- Multiple cellular compartments engagement in Nicotiana benthamiana-peanut stunt virus-satRNA interactions revealed by systems biology approach.Plant cell reports · 2021Article
- Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network.Frontiers in plant science · 2021Article
- Dynamic architecture and regulatory implications of the miRNA network underlying the response to stress in melon.RNA biology · 2020Article
- Genome-wide analysis of small RNAs from Odontoglossum ringspot virus and Cymbidium mosaic virus synergistically infecting Phalaenopsis.Molecular plant pathology · 2020Article
- Inferring the regulatory network of the miRNA-mediated response to biotic and abiotic stress in melon.BMC plant biology · 2019Article
- Alternative processing of its precursor is related to miR319 decreasing in melon plants exposed to cold.Scientific reports · 2018Article
- Article
- Dissecting the multifunctional role of the N-terminal domain of the Melon necrotic spot virus coat protein in RNA packaging, viral movement and interference with antiviral plant defence.Molecular plant pathology · 2017Article
- Article
- Interplays between Soil-Borne Plant Viruses and RNA Silencing-Mediated Antiviral Defense in Roots.Frontiers in microbiology · 2016Review
- Next-Generation Sequencing and Genome Editing in Plant Virology.Frontiers in microbiology · 2016Review
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundIn plants, RNA silencing plays a fundamental role as defence mechanism against viruses. During last years deep-sequencing technology has allowed to analyze the sRNA profile of a large variety of virus-infected tissues. Nevertheless, the majority of these studies have been restricted to a unique tissue and no comparative analysis between phloem and source/sink tissues has been conducted. In the present work, we compared the sRNA populations of source, sink and conductive (phloem) tissues in two different plant virus pathosystems. We chose two cucurbit species infected with two viruses very different in genome organization and replication strategy; Melon necrotic spot virus (MNSV) and Prunus necrotic ringspot virus (PNRSV).
resultsOur findings showed, in both systems, an increase of the 21-nt total sRNAs together with a decrease of those with a size of 24-nt in all the infected tissues, except for the phloem where the ratio of 21/24-nt sRNA species remained constant. Comparing the vsRNAs, both PNRSV- and MNSV-infected plants share the same vsRNA size distribution in all the analyzed tissues. Similar accumulation levels of sense and antisense vsRNAs were observed in both systems except for roots that showed a prevalence of (+) vsRNAs in both pathosystems. Additionally, the presence of overrepresented discrete sites along the viral genome, hot spots, were identified and validated by stem-loop RT-PCR. Despite that in PNRSV-infected plants the presence of vsRNAs was scarce both viruses modulated the host sRNA profile.
conclusionsWe compare for the first time the sRNA profile of four different tissues, including source, sink and conductive (phloem) tissues, in two plant-virus pathosystems. Our results indicate that antiviral silencing machinery in melon and cucumber acts mainly through DCL4. Upon infection, the total sRNA pattern in phloem remains unchanged in contrast to the rest of the analyzed tissues indicating a certain tissue-tropism to this polulation. Independently of the accumulation level of the vsRNAs both viruses were able to modulate the host sRNA pattern.
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