ArticleBMC genomics2015
Identification of sex-linked SNP markers using RAD sequencing suggests ZW/ZZ sex determination in Pistacia vera L.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 99 citations in OpenAlex.
- Investigating Sex-Biased Dispersal in a Vulnerable Marine Invertebrate, the European Spiny Lobster (Ecology and evolution · 2026Article
- Repeatome Dynamics and Sex Chromosome Differentiation in the XY and XYInternational journal of molecular sciences · 2025Article
- Unlocking Amborella's ZW sex chromosome system.Nature plants · 2024Article
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- Gender specific SNP markers in Coscinium fenestratum (Gaertn.) Colebr. for resource augmentation.Molecular biology reports · 2024Article
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- Development of sex-linked markers for gender identification of Actinidia arguta.Scientific reports · 2023Article
- Pharmacological Properties of Chemically Characterized Extracts from Mastic Tree: In Vitro and In Silico Assays.Life (Basel, Switzerland) · 2023Article
- Pistachio genomes provide insights into nut tree domestication and ZW sex chromosome evolution.Plant communications · 2023Article
- Evolution of sex in crops: recurrent scrap and rebuild.Breeding science · 2023Article
- Genome assembly and association tests identify interacting loci associated with vigor, precocity, and sex in interspecific pistachio rootstocks.G3 (Bethesda, Md.) · 2023Article
- DNA Methylation Difference between Female and Male Ussuri Catfish (Life (Basel, Switzerland) · 2022Article
- Article
- Genome-wide association links candidate genes to fruit firmness, fruit flesh color, flowering time, and soluble solid content in apricot (Prunus armeniaca L.).Molecular biology reports · 2022Article
- Molecular sexual determinants in Pistacia genus by KASP assay.Molecular biology reports · 2022Article
- GWAS Reveal Novel Sex-Related Markers and Candidate Genes in Sea Urchin Mesocentrotus nudus.Marine biotechnology (New York, N.Y.) · 2022Article
- RADSex: A computational workflow to study sex determination using restriction site-associated DNA sequencing data.Molecular ecology resources · 2021Article
- The Mastic Tree (Food technology and biotechnology · 2020Article
- Sex chromosome and sex locus characterization in goldfish, Carassius auratus (Linnaeus, 1758).BMC genomics · 2020Article
- A genome-wide identification, characterization and functional analysis of salt-related long non-coding RNAs in non-model plant Pistacia vera L. using transcriptome high throughput sequencing.Scientific reports · 2020Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundPistachio (Pistacia vera L.) is a dioecious species that has a long juvenility period. Therefore, development of marker-assisted selection (MAS) techniques would greatly facilitate pistachio cultivar-breeding programs. The sex determination mechanism is presently unknown in pistachio. The generation of sex-linked markers is likely to reduce time, labor, and costs associated with breeding programs, and will help to clarify the sex determination system in pistachio.
resultsRestriction site-associated DNA (RAD) markers were used to identify sex-linked markers and to elucidate the sex determination system in pistachio. Eight male and eight female F1 progenies from a Pistacia vera L. Siirt × Bağyolu cross, along with the parents, were subjected to RAD sequencing in two lanes of a Hi-Seq 2000 sequencing platform. This generated 449 million reads, comprising approximately 37.7 Gb of sequences. There were 33,757 polymorphic single nucleotide polymorphism (SNP) loci between the parents. Thirty-eight of these, from 28 RAD reads, were detected as putative sex-associated loci in pistachio. Validation was performed by SNaPshot analysis in 42 mature F1 progenies and in 124 cultivars and genotypes in a germplasm collection. Eight loci could distinguish sex with 100% accuracy in pistachio. To ascertain cost-effective application of markers in a breeding program, high-resolution melting (HRM) analysis was performed; four markers were found to perfectly separate sexes in pistachio. Because of the female heterogamety in all candidate SNP loci, we report for the first time that pistachio has a ZZ/ZW sex determination system. As the reported female-to-male segregation ratio is 1:1 in all known segregating populations and there is no previous report of super-female genotypes or female heteromorphic chromosomes in pistachio, it appears that the WW genotype is not viable.
conclusionSex-linked SNP markers were identified and validated in a large germplasm and proved their suitability for MAS in pistachio. HRM analysis successfully validated the sex-linked markers for MAS. For the first time in dioecious pistachio, a female heterogamety ZW/ZZ sex determination system is suggested.
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