ArticleBMC genomics2015
RNA Seq analysis of the Eimeria tenella gametocyte transcriptome reveals clues about the molecular basis for sexual reproduction and oocyst biogenesis.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 156 citations in OpenAlex.
- A single-cell atlas ofbioRxiv : the preprint server for biology · 2026Article
- Genome-wide transcriptomic analysis of Toxoplasma gondii reveals stage-specific regulatory programs and metabolic adaptations driving oocyst sporulation.BMC genomics · 2026Article
- Long-term feeder cell-free cat intestinal organoid cultures to study Toxoplasma gondii's sexual development.Communications biology · 2026Article
- Increased caecal Intestinimonas abundance inhibits E. tenella gametogenesis via EtGFAT regulation and alleviates infection through immunity.Microbiome · 2026Article
- Toxoplasma gondii chromatin remodeler SWI/SNF controls parasite division and gene expression.Nature communications · 2025Article
- Comparative analysis of model antigen expression level driven by the microneme protein promoters in Eimeria tenella.Poultry science · 2025Article
- Cultivating the endogenous life cycle ofParasitology · 2025Article
- Regulation of the developmental programs in Toxoplasma by a novel SNF2L-containing chromatin remodeling complex.Nature communications · 2025Article
- Taxifolin regulates SLC31A1-mediated cuproptosis and tumor progression in hepatocellular carcinoma.Human cell · 2025Article
- Transcriptome-wide mapping of internal mRNA NParasites & vectors · 2024Article
- Article
- Global profiling of protein S-palmitoylation in the second-generation merozoites of Eimeria tenella.Parasitology research · 2024Article
- Cryptosporidium life cycle small molecule probing implicates translational repression and an Apetala 2 transcription factor in macrogamont differentiation.PLoS pathogens · 2024Article
- Effects of prebiotic (lactoferrin) and diclazuril on broiler chickens experimentally infected withFrontiers in veterinary science · 2024Article
- Molecular characterization of cDNA coding for 33.5 and 41 kDa oocyst and sporocyst proteins that are differentially regulated in different strains ofFrontiers in veterinary science · 2024Article
- RNA-Seq of Phenotypically DistinctPathogens (Basel, Switzerland) · 2023Article
- Comparative proteomic analysis of wall-forming bodies and oocyst wall reveals the molecular basis underlying oocyst wall formation in Eimeria necatrix.Parasites & vectors · 2023Article
- Proteomic Analysis of FractionatedInternational journal of molecular sciences · 2023Article
- Overexpression of Eimeria tenella Rhoptry Kinase 2 Induces Early Production of Schizonts.Microbiology spectrum · 2023Article
- Comparative transcriptome profiling of Eimeria tenella in various developmental stages and functional analysis of an ApiAP2 transcription factor exclusively expressed during sporogony.Parasites & vectors · 2023Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe protozoan Eimeria tenella is a common parasite of chickens, causing avian coccidiosis, a disease of on-going concern to agricultural industries. The high prevalence of E. tenella can be attributed to the resilient oocyst stage, which is transmitted between hosts in the environment. As in related Coccidia, development of the eimerian oocyst appears to be dependent on completion of the parasite's sexual cycle. RNA Seq transcriptome profiling offers insights into the mechanisms governing the biology of E. tenella sexual stages (gametocytes) and the potential to identify targets for blocking parasite transmission.
resultsComparisons between the sequenced transcriptomes of E. tenella gametocytes and two asexual developmental stages, merozoites and sporozoites, revealed upregulated gametocyte transcription of 863 genes. Many of these genes code for proteins involved in coccidian sexual biology, such as oocyst wall biosynthesis and fertilisation, and some of these were characterised in more depth. Thus, macrogametocyte-specific expression and localisation was confirmed for two proteins destined for incorporation into the oocyst wall, as well as for a subtilisin protease and an oxidoreductase. Homologues of an oocyst wall protein and oxidoreductase were found in the related coccidian, Toxoplasma gondii, and shown to be macrogametocyte-specific. In addition, a microgametocyte gamete fusion protein, EtHAP2, was discovered.
conclusionsThe need for novel vaccine candidates capable of controlling coccidiosis is rising and this panel of gametocyte targets represents an invaluable resource for development of future strategies to interrupt parasite transmission, not just in Eimeria but in other Coccidia, including Toxoplasma, where transmission blocking is a relatively unexplored strategy.
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