ArticlePloS one2015
Accumulation of squalene in a microalga Chlamydomonas reinhardtii by genetic modification of squalene synthase and squalene epoxidase genes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 70 citations in OpenAlex.
- Progress and prospects in metabolic engineering approaches for isoprenoid biosynthesis in microalgae.Biotechnology for biofuels and bioproducts · 2025Review
- Exploiting microalgal diversity for sterol production.Frontiers in plant science · 2025Review
- Microbial Squalene: A Sustainable Alternative for the Cosmetics and Pharmaceutical Industry - A Review.Engineering in life sciences · 2024Review
- Proteomic characterization of a lutein-hyperaccumulating Chlamydomonas reinhardtii mutant reveals photoprotection-related factors as targets for increasing cellular carotenoid content.Biotechnology for biofuels and bioproducts · 2023Article
- Bioprocess conditions and regulation factors to optimize squalene production in thraustochytrids.World journal of microbiology & biotechnology · 2023Review
- Expression ofGenes · 2023Article
- Farnesyl pyrophosphate compartmentalization in the green microalga Chlamydomonas reinhardtii during heterologous (E)-α-bisabolene production.Microbial cell factories · 2022Article
- Recent advances in the microbial production of squalene.World journal of microbiology & biotechnology · 2022Review
- Extraction of Squalene From Tea Leaves (Frontiers in nutrition · 2022Article
- Green Microalgae Strain Improvement for the Production of Sterols and Squalene.Plants (Basel, Switzerland) · 2021Article
- Strategies for enhancing terpenoids accumulation in microalgae.Applied microbiology and biotechnology · 2021Review
- Channeling of Carbon Flux Towards Carotenogenesis inFrontiers in microbiology · 2021Article
- Overexpression of Key Sterol Pathway Enzymes in Two Model Marine Diatoms Alters Sterol Profiles inPharmaceuticals (Basel, Switzerland) · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Review
- Corrigendum: Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities.Frontiers in bioengineering and biotechnology · 2019Article
- Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities.Frontiers in bioengineering and biotechnology · 2019Review
- Functional characterization of squalene synthase and squalene epoxidase inPlant direct · 2018Article
- Cloning and characterization of squalene synthase and cycloartenol synthase fromActa pharmaceutica Sinica. B · 2017Article
- Temporal change of photophobic step-up responses of Euglena gracilis investigated through motion analysis.PloS one · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several microalgae accumulate high levels of squalene, and as such provide a potentially valuable source of this useful compound. However, the molecular mechanism of squalene biosynthesis in microalgae is still largely unknown. We obtained the sequences of two enzymes involved in squalene synthesis and metabolism, squalene synthase (CrSQS) and squalene epoxidase (CrSQE), from the model green alga Chlamydomonas reinhardtii. CrSQS was functionally characterized by expression in Escherichia coli and CrSQE by complementation of a budding yeast erg1 mutant. Transient expression of CrSQS and CrSQE fused with fluorescent proteins in onion epidermal tissue suggested that both proteins were co-localized in the endoplasmic reticulum. CrSQS-overexpression increased the rate of conversion of 14C-labeled farnesylpyrophosphate into squalene but did not lead to over-accumulation of squalene. Addition of terbinafine caused the accumulation of squalene and suppression of cell survival. On the other hand, in CrSQE-knockdown lines, the expression level of CrSQE was reduced by 59-76% of that in wild-type cells, and significant levels of squalene (0.9-1.1 μg mg-1 cell dry weight) accumulated without any growth inhibition. In co-transformation lines with CrSQS-overexpression and CrSQE-knockdown, the level of squalene was not increased significantly compared with that in solitary CrSQE-knockdown lines. These results indicated that partial knockdown of CrSQE is an effective strategy to increase squalene production in C. reinhardtii cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.