ArticleNature protocols2015
Live imaging of axonal transport in Drosophila pupal brain explants.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- RNP components condense into repressive RNP granules in the aging brain.Nature communications · 2022Article
- Live-Imaging of Axonal Cargoes in Drosophila Brain Explants Using Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- High-Resolution Live Imaging of Axonal RNP Granules in Drosophila Pupal Brain Explants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Axon guidance at the spinal cord midline-A live imaging perspective.The Journal of comparative neurology · 2021Article
- Article
- CytoCensus, mapping cell identity and division in tissues and organs using machine learning.eLife · 2020Article
- The prion-like domain of Drosophila Imp promotes axonal transport of RNP granules in vivo.Nature communications · 2019Article
- Temporal Control of Axonal Transport: The Extreme Case of Organismal Ageing.Frontiers in cellular neuroscience · 2019Review
- Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture.Journal of visualized experiments : JoVE · 2018Article
- Analysis of axonal trafficking via a novel live-imaging technique reveals distinct hedgehog transport kinetics.Biology open · 2017Article
- Lissencephaly-1 dependent axonal retrograde transport of L1-type CAM Neuroglian in the adult drosophila central nervous system.PloS one · 2017Article
- A conceptual view at microtubule plus end dynamics in neuronal axons.Brain research bulletin · 2016Review
- Filopodial dynamics and growth cone stabilization in Drosophila visual circuit development.eLife · 2015Article
- Long term ex vivo culturing of Drosophila brain as a method to live image pupal brains: insights into the cellular mechanisms of neuronal remodeling.Frontiers in cellular neuroscience · 2015Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Axonal transport is essential for the initial growth, maintenance and synaptic plasticity of axons, and altered axonal transport has been observed in different models of neurodegenerative pathologies. Dissecting the mechanisms underlying axonal transport in developing or degenerating brains requires dynamic imaging of axonal cargo movement in living samples. Whereas methods exist to image axonal transport in Drosophila larval neurons, they are not suitable to follow this process during metamorphosis, when brains undergo extensive remodeling. Here we present a simple method that enables confocal imaging of both fast and slow axonal transport in Drosophila pupal brain explants. We describe how to prepare chambers adapted for live imaging, how to maintain brain explants under physiological conditions and how to monitor and quantitatively analyze the movement of fluorescently labeled cargoes. This protocol requires minimal equipment and is ideally suited for experiments that combine genetics, optogenetics and pharmacological approaches. The brains can be prepared for image acquisition in 1.5 h, and the protocol can be performed easily in any fly laboratory.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.