ArticleEvidence-based complementary and alternative medicine : eCAM2015
Inhibitory Effects of Chrysanthemum boreale Essential Oil on Biofilm Formation and Virulence Factor Expression of Streptococcus mutans.
Article in Evidence-based complementary and alternative medicine : eCAM, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
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- Six Spain Thymus essential oils composition analysis and their in vitro and in silico study against Streptococcus mutans.BMC complementary medicine and therapies · 2023Article
- Antibacterial activity of oregano essential oils against Streptococcus mutans in vitro and analysis of active components.BMC complementary medicine and therapies · 2023Article
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- β-Caryophyllene in the Essential Oil fromMolecules (Basel, Switzerland) · 2019Article
- Effects of a New Nano-Silver Fluoride-Containing Dentifrice on Demineralization of Enamel andInternational journal of dentistry · 2018Article
- Analysis of Small RNAs in Streptococcus mutans under Acid Stress-A New Insight for Caries Research.International journal of molecular sciences · 2016Article
- Effects of norspermidine on Pseudomonas aeruginosa biofilm formation and eradication.MicrobiologyOpen · 2016Article
- Antimicrobial Activity of the Essential Oil of Plectranthus neochilus against Cariogenic Bacteria.Evidence-based complementary and alternative medicine : eCAM · 2015Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the study was to evaluate the antibacterial activity of essential oil extracted from Chrysanthemum boreale (C. boreale) on Streptococcus mutans (S. mutans). To investigate anticariogenic properties, and bacterial growth, acid production, biofilm formation, bacterial adherence of S. mutans were evaluated. Then gene expression of several virulence factors was also evaluated. C. boreale essential oil exhibited significant inhibition of bacterial growth, adherence capacity, and acid production of S. mutans at concentrations 0.1-0.5 mg/mL and 0.25-0.5 mg/mL, respectively. The safranin staining and scanning electron microscopy results showed that the biofilm formation was also inhibited. The result of live/dead staining showed the bactericidal effect. Furthermore, real-time PCR analysis showed that the gene expression of some virulence factors such as gtfB, gtfC, gtfD, gbpB, spaP, brpA, relA, and vicR of S. mutans was significantly decreased in a dose dependent manner. In GC and GC-MS analysis, seventy-two compounds were identified in the oil, representing 85.42% of the total oil. The major components were camphor (20.89%), β-caryophyllene (5.71%), α-thujone (5.46%), piperitone (5.27%), epi-sesquiphellandrene (5.16%), α-pinene (4.97%), 1,8-cineole (4.52%), β-pinene (4.45%), and camphene (4.19%). These results suggest that C. boreale essential oil may inhibit growth, adhesion, acid tolerance, and biofilm formation of S. mutans through the partial inhibition of several of these virulence factors.
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Registered trials
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