ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2014
Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Comparative accuracy of typhoid diagnostic tools: A Bayesian latent-class network analysis.PLoS neglected tropical diseases · 2019Pooled it
- A Novel Loop-Mediated Isothermal Amplification (LAMP) Primer Set for Detecting the STY2879 Gene ofMicroorganisms · 2026Article
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Performance of Immunodiagnostic Tests for Typhoid Fever: A Systematic Review and Meta-Analysis.Pathogens (Basel, Switzerland) · 2021Review
- Article
- Classification ofMicroorganisms · 2021Article
- Development of a New LAMP Assay for the Detection ofFrontiers in veterinary science · 2021Article
- Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.Infection and drug resistance · 2020Review
- Instrument-Free and Visual Detection ofInternational journal of molecular sciences · 2019Article
- Rapid Detection Device forIndian journal of microbiology · 2018Article
- Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.Journal of applied microbiology · 2018Review
- Article
- Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis.BMC microbiology · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and -10 times more sensitive in detecting S. Typhi compared to the optimised conventional polymerase chain reaction (PCR) method.
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