ArticleScientific reports2015
In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 18 citations in OpenAlex.
- Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction.Journal of synchrotron radiation · 2023Article
- Three-dimensional optical trapping and orientation of microparticles for coherent X-ray diffraction imaging.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy.Nature communications · 2018Article
- Study of a Microfluidic Chip Integrating Single Cell Trap and 3D Stable Rotation Manipulation.Micromachines · 2016Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in vivo elemental imaging in a two-dimensional (2D) projection mode in free-standing biological microorganisms or single cells, present in their aqueous environment. Using the model organism Scrippsiella trochoidea, a first proof of principle experiment at beamline ID13 of the European Synchrotron Radiation Facility (ESRF) demonstrates the feasibility of the OT XRF methodology, which is applied to study mixture toxicity of Cu-Ni and Cu-Zn as a result of elevated exposure. We expect that the new OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability.
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Registered trials
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