ArticleBiophysical journal2015
Cytoskeletal pinning controls phase separation in multicomponent lipid membranes.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
- Ghosts on the Membrane: Cytoskeletal Pinning Influences Nanoscale Cell Membrane Organization.Biomolecules · 2026Article
- Critical roles of plasma membrane dynamics and heterogeneity at the early stage of antigen-stimulated FcεRI signaling in mast cells.Biochemical Society transactions · 2025Review
- A Microfluidic Platform for Actin-Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains.Small science · 2025Article
- Cholesterol- and actin-centered view of the plasma membrane: updating the Singer-Nicolson fluid mosaic model to commemorate its 50th anniversaryMolecular biology of the cell · 2023Article
- Sensitive and selective polymer condensation at membrane surface driven by positive co-operativity.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Ceramide structure dictates glycosphingolipid nanodomain assembly and function.Nature communications · 2021Article
- Plasma membrane nano-organization specifies phosphoinositide effects on Rho-GTPases and actin dynamics in tobacco pollen tubes.The Plant cell · 2021Article
- Structured environments foster competitor coexistence by manipulating interspecies interfaces.PLoS computational biology · 2021Article
- Oxygen Transport Parameter in Plasma Membrane of Eye Lens Fiber Cells by Saturation Recovery EPR.Applied magnetic resonance · 2021Article
- FtsZ Reorganization Facilitates Deformation of Giant Vesicles in Microfluidic Traps*.Angewandte Chemie (International ed. in English) · 2020Article
- Membrane-Associated Nucleobase-Functionalized β-Peptides (β-PNAs) Affecting Membrane Support and Lipid Composition.Chembiochem : a European journal of chemical biology · 2020Article
- Myelin-Associated MAL and PLP Are Unusual among Multipass Transmembrane Proteins in Preferring Ordered Membrane Domains.The journal of physical chemistry. B · 2020Article
- Lipid Rafts: Controversies Resolved, Mysteries Remain.Trends in cell biology · 2020Review
- Structured environments fundamentally alter dynamics and stability of ecological communities.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Fix Your Membrane Receptor Imaging: Actin Cytoskeleton and CD4 Membrane Organization Disruption by Chemical Fixation.Frontiers in immunology · 2019Article
- The actions of volatile anesthetics: a new perspective.Acta crystallographica. Section D, Structural biology · 2018Review
- Quantifying spatial and temporal variations of the cell membrane ultra-structure by bimFCS.Methods (San Diego, Calif.) · 2018Review
- It Pays To Be in Phase.Biochemistry · 2018Review
- Structural determinants and functional consequences of protein affinity for membrane rafts.Nature communications · 2017Article
- Size and mobility of lipid domains tuned by geometrical constraints.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We study the effect of a minimal cytoskeletal network formed on the surface of giant unilamellar vesicles by the prokaryotic tubulin homolog, FtsZ, on phase separation in freestanding lipid membranes. FtsZ has been modified to interact with the membrane through a membrane targeting sequence from the prokaryotic protein MinD. FtsZ with the attached membrane targeting sequence efficiently forms a highly interconnected network on membranes with a concentration-dependent mesh size, much similar to the eukaryotic cytoskeletal network underlying the plasma membrane. Using giant unilamellar vesicles formed from a quaternary lipid mixture, we demonstrate that the artificial membrane-associated cytoskeleton, on the one hand, suppresses large-scale phase separation below the phase transition temperature, and, on the other hand, preserves phase separation above the transition temperature. Our experimental observations support the ideas put forward in our previous simulation study: In particular, the picket fence effect on phase separation may explain why micrometer-scale membrane domains are observed in isolated, cytoskeleton-free giant plasma membrane vesicles, but not in intact cell membranes. The experimentally observed suppression of large-scale phase separation much below the transition temperatures also serves as an argument in favor of the cryoprotective role of the cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.