Evidence map›Paper›PMID 25762292›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Applying Shear Stress to Pluripotent Stem Cells.

Russell P Wolfe, Julia B Guidry, Stephanie L Messina, Tabassum Ahsan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Russell P WolfeDepartment of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA.
Julia B GuidryDepartment of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA.
Stephanie L MessinaDepartment of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA.
Tabassum AhsanDepartment of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA. tahsan@tulane.edu.

Funding

The role of the CMV-associated reactive oxygen species in age-relatedP20GM103629 · NIGMS · TULANE UNIVERSITY OF LOUISIANA · PI ROUT, NAMITA · 2012 to 2021
$22.4M
NIGMS NIH HHS P20 GM103629
6 · The paper itself

Abstract

Thorough understanding of the effects of shear stress on stem cells is critical for the rationale design of large-scale production of cell-based therapies. This is of growing importance as emerging tissue engineering and regenerative medicine applications drive the need for clinically relevant numbers of both pluripotent stem cells (PSCs) and cells derived from PSCs. Here, we describe the use of a custom parallel plate bioreactor system to impose fluid shear stress on a layer of PSCs adhered to protein-coated glass slides. This system can be useful both for basic science studies in mechanotransduction and as a surrogate model for bioreactors used in large-scale production.

Indexed as

BioreactorsAnimalsCell Culture TechniquesEmbryonic Stem CellsEquipment DesignHumansInduced Pluripotent Stem CellsMechanotransduction, CellularSterilizationStress, MechanicalBioreactorEmbryonic stem cellsInduced pluripotent stem cellsMechanotransductionPhysical microenvironmentShear stressStem cell differentiation

Identifiers

PMID25762292
PMCPMC5026703

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.