ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2015
Integration of computational modeling with membrane transport studies reveals new insights into amino acid exchange transport mechanisms.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Feasibility of Transport of 26 Biologically Active Ultrashort Peptides via LAT and PEPT Family Transporters.Biomolecules · 2023Article
- Do Amino Acid Antiporters Have Asymmetric Substrate Specificity?Biomolecules · 2023Article
- Heteromeric Amino Acid Transporters in Brain: from Physiology to Pathology.Neurochemical research · 2022Review
- Homocysteine Metabolism in Pregnancy and Developmental Impacts.Frontiers in cell and developmental biology · 2022Review
- Placenta-specific Slc38a2/SNAT2 knockdown causes fetal growth restriction in mice.Clinical science (London, England : 1979) · 2021Article
- Amino Acids and Developmental Origins of Hypertension.Nutrients · 2020Review
- Genome-wide identification and expression analysis of the AAAP family in Medicago truncatula.Genetica · 2019Article
- Blood flow and transport in the human placenta.Annual review of fluid mechanics · 2019Article
- A systems perspective on placental amino acid transport.The Journal of physiology · 2018Review
- Estrone sulphate uptake by the microvillous membrane of placental syncytiotrophoblast is coupled to glutamate efflux.Biochemical and biophysical research communications · 2018Article
- Treating the dysfunctional placenta.The Journal of endocrinology · 2017Review
- Computational modelling of placental amino acid transfer as an integrated system.Biochimica et biophysica acta · 2016Article
- Phenylalanine transfer across the isolated perfused human placenta: an experimental and modeling investigation.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
- Glutamate cycling may drive organic anion transport on the basal membrane of human placental syncytiotrophoblast.The Journal of physiology · 2015Article
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Authors and funding
10 authors.
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Abstract
Uptake of system L amino acid substrates into isolated placental plasma membrane vesicles in the absence of opposing side amino acid (zero-trans uptake) is incompatible with the concept of obligatory exchange, where influx of amino acid is coupled to efflux. We therefore hypothesized that system L amino acid exchange transporters are not fully obligatory and/or that amino acids are initially present inside the vesicles. To address this, we combined computational modeling with vesicle transport assays and transporter localization studies to investigate the mechanisms mediating [(14)C]L-serine (a system L substrate) transport into human placental microvillous plasma membrane (MVM) vesicles. The carrier model provided a quantitative framework to test the 2 hypotheses that l-serine transport occurs by either obligate exchange or nonobligate exchange coupled with facilitated transport (mixed transport model). The computational model could only account for experimental [(14)C]L-serine uptake data when the transporter was not exclusively in exchange mode, best described by the mixed transport model. MVM vesicle isolates contained endogenous amino acids allowing for potential contribution to zero-trans uptake. Both L-type amino acid transporter (LAT)1 and LAT2 subtypes of system L were distributed to MVM, with L-serine transport attributed to LAT2. These findings suggest that exchange transporters do not function exclusively as obligate exchangers.
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