ArticlePloS one2015
Identical assemblage of Giardia duodenalis in humans, animals and vegetables in an urban area in southern Brazil indicates a relationship among them.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Giardiasis as a neglected disease in Brazil: Systematic review of 20 years of publications.PLoS neglected tropical diseases · 2017Pooled it
- Human Giardiasis in Ghana - A Scoping Review of Studies From 2004 to 2024.Health science reports · 2025Article
- Assessment of potential zoonotic transmission ofOne health (Amsterdam, Netherlands) · 2023Review
- Role of rodents in the zoonotic transmission of giardiasis.One health (Amsterdam, Netherlands) · 2023Review
- Giardia duodenalis in Hu sheep: occurrence and environmental contamination on large-scale housing farms.Parasite (Paris, France) · 2023Article
- Review
- Detection ofFood and waterborne parasitology · 2022Article
- Detection ofMicroorganisms · 2022Review
- Molecular characterisation ofCurrent research in parasitology & vector-borne diseases · 2022Article
- Giardiasis in urban and rural Amazonas, Brazil is driven by zoonotic and cosmopolitan A and B assemblages.Memorias do Instituto Oswaldo Cruz · 2022Article
- Standardization of molecular techniques for the detection and characterization of intestinal protozoa and other pathogens in humans.The journal of venomous animals and toxins including tropical diseases · 2022Article
- Comparative analysis of routine parasitological methods for recovery of cysts, molecular detection, and genotyping of Giardia duodenalis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Multilocus Genotyping ofPathogens (Basel, Switzerland) · 2021Article
- Detection of Cryptosporidium spp. and Giardia duodenalis in small wild mammals in northeastern Brazil.PloS one · 2021Article
- Epidemiology of Giardia duodenalis assemblages in Brazil: there is still a long way to go.Memorias do Instituto Oswaldo Cruz · 2021Review
- Molecular Characterization ofMicroorganisms · 2020Article
- The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil.Biomedica : revista del Instituto Nacional de Salud · 2020Article
- Detection of human intestinal protozoan parasites in vegetables and fruits: a review.Parasites & vectors · 2020Review
- Insights about the structure of farnesyl diphosphate synthase (FPPS) and the activity of bisphosphonates on the proliferation and ultrastructure of Leishmania and Giardia.Parasites & vectors · 2020Article
- Improvement in cyst recovery and molecular detection of Giardia duodenalis from stool samples.Molecular biology reports · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGiardia duodenalis infects humans and other mammals by ingestion of cysts in contaminated water or food, or directly in environments with poor hygiene. Eight assemblages, designated A-H, are described for this species. METHODOLOGY/PRINCIPAL
findingsWe investigated by microscopy or by direct immunofluorescence technique the occurrence of G. duodenalis in 380 humans, 34 animals, 44 samples of water and 11 of vegetables. G. duodenalis cysts present in samples were genotyped through PCR-RFLP of β giardin and glutamate dehydrogenase (gdh) genes and sequencing of gdh. The gdh gene was amplified in 76.5% (26/34) of the human faeces samples with positive microscopy and in 2.9% (1/34) of negative samples. In 70.4% (19/27) of the positive samples were found BIV assemblage. In two samples from dogs with positive microscopy and one negative sample, assemblages BIV, C, and D were found. Cysts of Giardia were not detected in water samples, but three samples used for vegetable irrigation showed total coliforms above the allowed limit, and Escherichia coli was observed in one sample. G. duodenalis BIV was detected in two samples of Lactuca sativa irrigated with this sample of water. BIV was a common genotype, with 100% similarity, between different sources or hosts (humans, animals and vegetables), and the one most often found in humans. CONCLUSIONS/SIGNIFICANCE: This is the first study in Brazil that reports the connection among humans, dogs and vegetables in the transmission dynamics of G. duodenalis in the same geographic area finding identical assemblage. BIV assemblage was the most frequently observed among these different links in the epidemiological chain.
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