ArticlePloS one2015
A millifluidic study of cell-to-cell heterogeneity in growth-rate and cell-division capability in populations of isogenic cells of Chlamydomonas reinhardtii.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Low-Frequency Segmented-Flow Microfluidics for Biphasic Chemical Sensing.Sensors and actuators. B, Chemical · 2026Article
- Interplay between high-energy quenching and state transitions in Chlamydomonas reinhardtii: a single-cell approach.The New phytologist · 2026Article
- Phenotypic heterogeneity in a batch culture of Chlamydomonas reinhardtii with different light tolerances.PloS one · 2026Article
- Impact of micro-habitat fragmentation on microbial population growth dynamics.The ISME journal · 2025Article
- Structured population balances to support microalgae-based processes: Review of the state-of-art and perspectives analysis.Computational and structural biotechnology journal · 2023Review
- Tracking the stochastic growth of bacterial populations in microfluidic droplets.Physical biology · 2022Article
- Long-term survival of Chlamydomonas reinhardtii during conditional senescence.Archives of microbiology · 2021Article
- In Silico and Cellular Differences Related to the Cell Division Process between the A and B Races of the Colonial MicroalgaBiomolecules · 2021Article
- Single-cell RNA sequencing of batch Chlamydomonas cultures reveals heterogeneity in their diurnal cycle phase.The Plant cell · 2021Article
- Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular CyanobacteriumFrontiers in microbiology · 2021Article
- Article
- Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology.Micromachines · 2019Review
- Microfluidic techniques for enhancing biofuel and biorefinery industry based on microalgae.Biotechnology for biofuels · 2019Article
- Sizing up the cell cycle: systems and quantitative approaches in Chlamydomonas.Current opinion in plant biology · 2018Review
- Article
- Review: Microbial analysis in dielectrophoretic microfluidic systems.Analytica chimica acta · 2017Review
- Single-Cell Growth Rates in Photoautotrophic Populations Measured by Stable Isotope Probing and Resonance Raman Microspectrometry.Frontiers in microbiology · 2017Article
- Ecotoxicity assessment using ciliate cells in millifluidic droplets.Biomicrofluidics · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To address possible cell-to-cell heterogeneity in growth dynamics of isogenic cell populations of Chlamydomonas reinhardtii, we developed a millifluidic drop-based device that not only allows the analysis of populations grown from single cells over periods of a week, but is also able to sort and collect drops of interest, containing viable and healthy cells, which can be used for further experimentation. In this study, we used isogenic algal cells that were first synchronized in mixotrophic growth conditions. We show that these synchronized cells, when placed in droplets and kept in mixotrophic growth conditions, exhibit mostly homogeneous growth statistics, but with two distinct subpopulations: a major population with a short doubling-time (fast-growers) and a significant subpopulation of slowly dividing cells (slow-growers). These observations suggest that algal cells from an isogenic population may be present in either of two states, a state of restricted division and a state of active division. When isogenic cells were allowed to propagate for about 1000 generations on solid agar plates, they displayed an increased heterogeneity in their growth dynamics. Although we could still identify the original populations of slow- and fast-growers, drops inoculated with a single progenitor cell now displayed a wider diversity of doubling-times. Moreover, populations dividing with the same growth-rate often reached different cell numbers in stationary phase, suggesting that the progenitor cells differed in the number of cell divisions they could undertake. We discuss possible explanations for these cell-to-cell heterogeneities in growth dynamics, such as mutations, differential aging or stochastic variations in metabolites and macromolecules yielding molecular switches, in the light of single-cell heterogeneities that have been reported among isogenic populations of other eu- and prokaryotes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.