Evidence map›Paper›PMID 25760593›Full record

ArticleActa crystallographica. Section D, Biological crystallography2015

Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.

Mark Stauber, Jean Jakoncic, Jacob Berger, Jerome M Karp, Ariel Axelbaum, Dahniel Sastow, Sergey V Buldyrev, Bruce J Hrnjez, Neer Asherie

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section D, Biological crystallography, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Mark StauberDepartment of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
Jean JakoncicNational Synchrotron Light Source, Brookhaven National Laboratory, Building 725D, Upton, NY 11973-5000, USA.
Jacob BergerDepartment of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
Jerome M KarpDepartment of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
Ariel AxelbaumDepartment of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
Dahniel SastowDepartment of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
Sergey V BuldyrevDepartment of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
Bruce J HrnjezCollegiate School, 260 West 78th Street, New York, NY 10024-6559, USA.
Neer AsherieDepartment of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
Yeshiva University · USBrookhaven National Laboratory · US

Funding

NIGMS NIH HHS Y01 GM000080
6 · The paper itself

Abstract

Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.

Indexed as

Crystallography, X-RayGlycolsMuramidaseProtein Structure, TertiaryGlycolshexylene glycolMuramidasechiralitycrystallization additiveshigh-resolution protein structuresMPDprecipitants

Identifiers

PMID25760593
PMCPMC4356360
OpenAlexW2051468379

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.