Evidence map›Paper›PMID 25760267›Full record

ArticleJournal of applied oral science : revista FOB

Novel biological activity of ameloblastin in enamel matrix derivative.

Sachiko Kuramitsu-Fujimoto, Wataru Ariyoshi, Noriko Saito, Toshinori Okinaga, Masaharu Kamo, Akira Ishisaki, Takashi Takata, Kazunori Yamaguchi, Tatsuji Nishihara

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of applied oral science : revista FOB. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Sachiko Kuramitsu-FujimotoDivision of Orofacial Functions and Orthodontics, Department of Growth Development of Functions, Kyushu Dental University, Fukuoka, Japan.
Wataru AriyoshiDivision of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Fukuoka, Japan.
Noriko SaitoDivision of Pulp Biology, Operative Dentistry and Endodontics, Department of Cariology and Periodontology, Kyushu Dental University, Fukuoka, Japan.
Toshinori OkinagaDivision of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Fukuoka, Japan.
Masaharu KamoDivision of Cellular Biosignal Sciences, Department of Biochemistry, Iwate Medical University, Iwate, Japan.
Akira IshisakiDivision of Cellular Biosignal Sciences, Department of Biochemistry, Iwate Medical University, Iwate, Japan.
Takashi TakataDepartment of Oral and Maxillofacial Pathobiology, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Kazunori YamaguchiDivision of Orofacial Functions and Orthodontics, Department of Growth Development of Functions, Kyushu Dental University, Fukuoka, Japan.
Tatsuji NishiharaDivision of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University, Fukuoka, Japan.
Kyushu Dental University · JPIwate Medical University · JPHiroshima University of Economics · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveEnamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration. However, the effects of EMD on gingival epithelial cells during regeneration of periodontal tissues are unclear. In this in vitro study, we purified ameloblastin from EMD and investigated its biological effects on epithelial cells. MATERIAL AND

methodsBioactive fractions were purified from EMD by reversed-phase high-performance liquid chromatography using hydrophobic support with a C18 column. The mouse gingival epithelial cell line GE-1 and human oral squamous cell carcinoma line SCC-25 were treated with purified EMD fraction, and cell survival was assessed with a WST-1 assay. To identify the proteins in bioactive fractions of EMD, we used proteome analysis with two-dimensional gel electrophoresis followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.

resultsPurified fractions from EMD suppressed proliferation of GE-1 and SCC-25. LC-MS/MS revealed that ameloblastin in EMD is the component responsible for inhibiting epithelial cell proliferation. The inhibitory effect of ameloblastin on the proliferation of GE-1 and SCC-25 was confirmed using recombinant protein.

conclusionThe inhibitory effects of EMD on epithelial cell proliferation are caused by the biological activities of ameloblastin, which suggests that ameloblastin is involved in regulating epithelial downgrowth in periodontal tissues.

Indexed as

AnimalsCell ProliferationCells, CulturedCell SurvivalChromatography, High Pressure LiquidDental Enamel ProteinsElectrophoresis, Gel, Two-DimensionalEpithelial CellsGingivaGuided Tissue Regeneration, PeriodontalHumansMicePeriodontitisPeriodontiumReference ValuesReproducibility of ResultsAMBN protein, humanDental Enamel Proteinsenamel matrix proteins

Identifiers

PMID25760267
PMCPMC4349119
OpenAlexW1976580351

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.