ArticleeLife2015
2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
What it found
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Who cites it
98 citing papers in PubMed, 184 citations in OpenAlex.
- Article
- AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.Acta crystallographica. Section D, Structural biology · 2026Article
- Ultra-rapid cryo-EM data acquisition method enabled by continuous recording based beam image shift.Nature communications · 2026Article
- Exploring Novel E3 Ligases and Neosubstrates for Molecular Glue Degraders and Therapeutic Applications in Cancer.Oncology research · 2026Review
- ANTIDOTE: A Metadata-Driven Neural Network for Improving CryoEM 3-D Particle Sorting.bioRxiv : the preprint server for biology · 2025Article
- Article
- Self-supervised machine learning framework for high-throughput electron microscopy.Science advances · 2025Article
- Complex Water Networks Visualized through 2.2-2.3 Å Cryogenic Electron Microscopy of RNA.bioRxiv : the preprint server for biology · 2025Article
- Factors affecting macromolecule orientations in thin films formed in cryo-EM.Acta crystallographica. Section D, Structural biology · 2024Article
- Classification of helical polymers with deep-learning language models.Journal of structural biology · 2023Article
- Cryo-EM for Small Molecules.Current protocols · 2022Review
- Article
- IceBreaker: Software for high-resolution single-particle cryo-EM with non-uniform ice.Structure (London, England : 1993) · 2022Article
- Cryo-electron tomography related radiation-damage parameters for individual-molecule 3D structure determination.Frontiers in chemistry · 2022Review
- Analysis and comparison of electron radiation damage assessments in Cryo-EM by single particle analysis and micro-crystal electron diffraction.Frontiers in molecular biosciences · 2022Review
- High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy.The Review of scientific instruments · 2021Article
- DRPnet: automated particle picking in cryo-electron micrographs using deep regression.BMC bioinformatics · 2021Article
- FSC-Q: a CryoEM map-to-atomic model quality validation based on the local Fourier shell correlation.Nature communications · 2021Article
- Topaz-Denoise: general deep denoising models for cryoEM and cryoET.Nature communications · 2020Article
- High-Throughput Cryo-EM Enabled by User-Free Preprocessing Routines.Structure (London, England : 1993) · 2020Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Recent developments in detector hardware and image-processing software have revolutionized single particle cryo-electron microscopy (cryoEM) and led to a wave of near-atomic resolution (typically ∼3.3 Å) reconstructions. Reaching resolutions higher than 3 Å is a prerequisite for structure-based drug design and for cryoEM to become widely interesting to pharmaceutical industries. We report here the structure of the 700 kDa Thermoplasma acidophilum 20S proteasome (T20S), determined at 2.8 Å resolution by single-particle cryoEM. The quality of the reconstruction enables identifying the rotameric conformation adopted by some amino-acid side chains (rotamers) and resolving ordered water molecules, in agreement with the expectations for crystal structures at similar resolutions. The results described in this manuscript demonstrate that single particle cryoEM is capable of competing with X-ray crystallography for determination of protein structures of suitable quality for rational drug design.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.