ArticleBiomicrofluidics2015
Single-cell printing based on impedance detection.
Article in Biomicrofluidics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Droplet-based bioprinting.Nature reviews. Methods primers · 2025Article
- Single-Cell Isolation Microfluidic Chip Based on Thermal Bubble Micropump Technology.Sensors (Basel, Switzerland) · 2023Article
- Nucleic Acid Quantification by Multi-Frequency Impedance Cytometry and Machine Learning.Biosensors · 2023Article
- Efficient Cell Impedance Measurement by Dielectrophoretic Cell Accumulation and Evaluation of Chondrogenic Phenotypes.Micromachines · 2022Article
- Advances in Single-Cell Printing.Micromachines · 2022Review
- What is the future of electrical impedance spectroscopy in flow cytometry?Biomicrofluidics · 2021Article
- Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.Lab on a chip · 2021Article
- The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves.MethodsX · 2021Article
- Single Cell Metabolomics: A Future Tool to Unmask Cellular Heterogeneity and Virus-Host Interaction in Context of Emerging Viral Diseases.Frontiers in microbiology · 2020Review
- Droplet encapsulation of particles in different regimes and sorting of particle-encapsulating-droplets from empty droplets.Biomicrofluidics · 2019Article
- Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Functional TCR T cell screening using single-cell droplet microfluidics.Lab on a chip · 2018Article
- Dotette: Programmable, high-precision, plug-and-play droplet pipetting.Biomicrofluidics · 2018Article
- An open-pattern droplet-in-oil planar array for single cell analysis based on sequential inkjet printing technology.Biomicrofluidics · 2017Article
- Deterministic bead-in-droplet ejection utilizing an integrated plug-in bead dispenser for single bead-based applications.Scientific reports · 2017Article
- Improvement of Depth Profiling into Biotissues Using Micro Electrical Impedance Spectroscopy on a Needle with Selective Passivation.Sensors (Basel, Switzerland) · 2016Article
- Micro electrical impedance spectroscopy on a needle for ex vivo discrimination between human normal and cancer renal tissues.Biomicrofluidics · 2016Article
- Spatial concentration distribution analysis of cells in electrode-multilayered microchannel by dielectric property measurement.Biomicrofluidics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Label-free isolation of single cells is essential for the growing field of single-cell analysis. Here, we present a device which prints single living cells encapsulated in free-flying picoliter droplets. It combines inkjet printing and impedance flow cytometry. Droplet volume can be controlled in the range of 500 pl-800 pl by piezo actuator displacement. Two sets of parallel facing electrodes in a 50 μm × 55 μm channel are applied to measure the presence and velocity of a single cell in real-time. Polystyrene beads with <5% variation in diameter generated signal variations of 12%-17% coefficients of variation. Single bead efficiency (i.e., printing events with single beads vs. total number of printing events) was 73% ± 11% at a throughput of approximately 9 events/min. Viability of printed HeLa cells and human primary fibroblasts was demonstrated by culturing cells for at least eight days.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.