Sequencing and Gene Expression Analysis of Leishmania tropica LACK Gene.

Nour Hammoudeh, Mahmoud Kweider, Abdul-Qader Abbady, Chadi Soukkarieh

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Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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1 citing paper in PubMed, 1 citations in OpenAlex.

Genetic Diversity and Phylogenetic Study ofIranian journal of parasitology
Article

4 · The record

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5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Nour HammoudehDept. of Biotechnology, Faculty of Agriculture, Damascus University, Damascus, Syria.

Mahmoud KweiderDept. of Animal Biology, Faculty of Sciences, Damascus University, Damascus, Syria.

Abdul-Qader AbbadyDept. of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria(AECS), Damascus, Syria.

Chadi SoukkariehDept. of Animal Biology, Faculty of Sciences, Damascus University, Damascus, Syria.

Damascus University · SYAtomic Energy Commission of Syria · SY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLeishmania Homologue of receptors for Activated C Kinase (LACK) antigen is a 36-kDa protein, which provokes a very early immune response against Leishmania infection. There are several reports on the expression of LACK through different life-cycle stages of genus Leishmania, but only a few of them have focused on L.tropica.

methodsThe present study provides details of the cloning, DNA sequencing and gene expression of LACK in this parasite species. First, several local isolates of Leishmania parasites were typed in our laboratory using PCR technique to verify of Leishmania parasite species. After that, LACK gene was amplified and cloned into a vector for sequencing. Finally, the expression of this molecule in logarithmic and stationary growth phase promastigotes, as well as in amastigotes, was evaluated by Reverse Transcription-PCR (RT-PCR) technique.

resultsThe typing result confirmed that all our local isolates belong to L.tropica. LACK gene sequence was determined and high similarity was observed with the sequences of other Leishmania species. Furthermore, the expression of LACK gene in both promastigotes and amastigotes forms was confirmed.

conclusionOverall, the data set the stage for future studies of the properties and immune role of LACK gene products.

Indexed as

AmastigotesCutaneous leishmaniasisLACK geneLeishmania tropicaPromastigotes

Identifiers

PMID25759740

PMCPMC4345098

OpenAlexW2134604220

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.