ArticleWorld journal of gastroenterology2015
Isolation and diagnosis of Helicobacter pylori by a new method: microcapillary culture.
Article in World journal of gastroenterology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Helicobacter pylori in Turkish Children with Dyspepsia: Diagnosis, Prevalence, Genotyping and Antibiotic Resistance.Indian journal of pediatrics · 2026Article
- Evaluation of a Specific Modulation Frequency Technique for DetectingArchives of Razi Institute · 2022Article
- Chinese Consensus Report on Family-BasedGut · 2022Article
- Resuscitation of the Helicobacter pylori Coccoid Forms by Resuscitation Promoter Factor Obtained from Micrococcus Luteus.Current microbiology · 2020Article
- First-time serological and molecular detection ofVeterinary world · 2018Article
- Diagnosis ofJournal of pathogens · 2018Review
- Helicobacter Pylori Infection: Diagnostic Strategies in Primary Diagnosis and After Therapy.Digestive diseases and sciences · 2016Review
- Diagnostic and Treatment Approaches for Refractory Peptic Ulcers.Clinical endoscopy · 2015Review
- Real-time PCR detection ofHippokratiaArticle
- Hybrid Therapy versus Sequential Therapy for Eradication ofJournal of pharmacology & pharmacotherapeuticsArticle
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Authors and funding
15 authors at 1 institution in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the performance of the microcapillary culture method (MCM) in Helicobacter pylori (H. pylori) isolation and diagnosis.
methodsMicrocapillary culture (MC), classical culture (CC), rapid urease (CLO) test, and histopathologic examination (HE) were performed with biopsy samples. Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 °C without providing a microaerophilic environment. Additionally, three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium (Becton Dickinson, Helicobacter Agar, Modified) specific for the isolation of H. pylori and incubated at 37 °C in a microaerophilic atmosphere provided by CampyGen (Becton Dickinson, GasPack). Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content. Results obtained by CC, CLO test, and HE were compared with those of MC. The diagnostic performances of the methods used in this study were evaluated for specificity, sensitivity, positive predictive value (PPV), negative predictive value (NPV), and CI.
resultsH. pylori was found positive by CLO test + HE and/or CC culture in 26 patient antrum and corpus biopsy samples. In 25 (25/26) patient biopsy samples, H. pylori was isolated by MCM, whereas in only 14 (14/26) patient biopsy samples, H. pylori was isolated by CC. CLO test and HE were found positive in 17 (17/26) patient biopsy samples. Comparing the results of the isolation of H. pylori by MCM, CC, CLO test, and HE, the sensitivity of the MCM was found as 96%, the specificity as 80%, the PPV as 83%, the NPV as 95%, and the 95%CI as 0.76 (χ (2) = 31.51, P < 0.01) whereas the sensitivity of the CC was found as 54% (χ (2) = 19.15, P < 0.01), and the sensitivity of the CLO test and HE were found as 65% (χ (2) = 25.26, P < 0.01).
conclusionThis new microcapillary cultivation method for H. pylori has high diagnostic sensitivity compared with CC, HE, and CLO tests.
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