ArticleThe American journal of tropical medicine and hygiene2015
The Effect of Storage and Extraction Methods on Amplification of Plasmodium falciparum DNA from Dried Blood Spots.
Article in The American journal of tropical medicine and hygiene, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 54 citations in OpenAlex.
- Targeting malaria in high-risk populations in low endemic regions in northern Namibia: a quasi-experimental controlled trial to reduce malaria in seasonal agricultural workers and cattle herders.BMJ global health · 2025Trial
- Comparative evaluation of multiplex bead assay, polymerase chain reaction, and rapid diagnostic test for P. falciparum detection in community surveys in Malawi.Malaria journal · 2026Article
- Community reservoirs of malaria parasites and gametocytes in Arba Minch district, southern Rift Valley, Ethiopia: a cross-sectional study.Malaria journal · 2026Article
- Coinheritance of polymorphic alleles ofOne health (Amsterdam, Netherlands) · 2025Article
- Evaluation of dried blood spot sampling for real-time PCR malaria diagnostics in a rural setting in Angola.Parasites & vectors · 2025Article
- Forest-goers as a heterogeneous population at high-risk for malaria: a case-control study in Aceh Province, Indonesia.Malaria journal · 2024Article
- Malaria elimination in Ghana: recommendations for reactive case detection strategy implementation in a low endemic area of Asutsuare, Ghana.Malaria journal · 2024Review
- A cross-sectional study to ascertain malaria prevalence among asymptomatic travellers arriving on the Lihir Group of Islands, Papua New Guinea: implications for elimination efforts.Malaria journal · 2023Article
- Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era.PLoS neglected tropical diseases · 2023Article
- Malaria misdiagnosis in the routine health system in Arba Minch area district in southwest Ethiopia: an implication for malaria control and elimination.Malaria journal · 2023Article
- Rapid Nucleic Acid Extraction for Aquatic Animal DNA Virus Determination Using Chelex 100 Resin via Conventional PCR and Digital Droplet PCR Detection.Animals : an open access journal from MDPI · 2022Article
- Colorimetric and Real-Time Loop-Mediated Isothermal Amplification (LAMP) for Detection ofDiagnostics (Basel, Switzerland) · 2022Article
- Tenfold difference in DNA recovery rate: systematic comparison of whole blood vs. dried blood spot sample collection for malaria molecular surveillance.Malaria journal · 2022Article
- Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda.Malaria journal · 2022Article
- An algorithm based on molecular protocols to improve the detection of Plasmodium in autochthonous malarial areas in the Atlantic Forest biome.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- Preservation and Extraction of Malaria Parasite DNA from Dried Blood Spots.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Zoonotic Blood-Borne Pathogens in Non-Human Primates in the Neotropical Region: A Systematic Review.Pathogens (Basel, Switzerland) · 2021Review
- Template copy number and the sensitivity of quantitative PCR for Plasmodium falciparum in asymptomatic individuals.Malaria journal · 2020Article
- Malaria infection prevalence and sensitivity of reactive case detection in Zanzibar.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2020Article
- Active Case Finding for Malaria: A 3-Year National Evaluation of Optimal Approaches to Detect Infections and Hotspots Through Reactive Case Detection in the Low-transmission Setting of Eswatini.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Extraction and amplification of DNA from dried blood spots (DBS) collected in field studies is commonly used for detection of Plasmodium falciparum. However, there have been few systematic efforts to determine the effects of storage and extraction methods on the sensitivity of DNA amplification. We investigated the effects of storage conditions, length of storage, and DNA extraction methods on amplification via three PCR-based assays using field samples and laboratory controls. Samples stored as DBS for 2 or more years at ambient temperature showed a significant loss of sensitivity that increased with time; after 10 years only 10% samples with parasite densities > 1,000 parasites/μL were detectable by nested polymerase chain reaction (PCR). Conversely, DBS and extracted DNA stored at -20°C showed no loss of sensitivity with time. Samples with low parasite densities amplified more successfully with saponin/Chelex compared with spin-column-based extraction, though the latter method performed better on samples with higher parasite densities stored for 2 years at ambient temperature. DNA extracted via both methods was stable after 20 freeze-thaw cycles. Our results suggest that DBS should be stored at -20°C or extracted immediately, especially if anticipating 2 or more years of storage.
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