ArticleBioTechniques2015
Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.
Article in BioTechniques, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed.
- O-Mannose Glycosylations Influence E-Cadherin Functional Interactions.Molecular & cellular proteomics : MCP · 2026Article
- Article
- Multiparameter screen optimizes immunoprecipitation.BioTechniques · 2024Article
- Interrogating data-independent acquisition LC-MS/MS for affinity proteomics.Journal of proteins and proteomics · 2024Article
- 7SK methylation by METTL3 promotes transcriptional activity.Science advances · 2023Article
- Affinity-Based Interactome Analysis of Endogenous LINE-1 Macromolecules.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Bringing synapses into focus: Recent advances in synaptic imaging and mass-spectrometry for studying synaptopathy.Frontiers in synaptic neuroscience · 2023Review
- Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Chromatography-Free Purification Strategies for Large Biological Macromolecular Complexes Involving Fractionated PEG Precipitation and Density Gradients.Life (Basel, Switzerland) · 2021Review
- Affinity proteomic dissection of the human nuclear cap-binding complex interactome.Nucleic acids research · 2020Article
- Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Immunoprecipitation for the Analysis of Macromolecular Complexes in Trypanosoma cruzi.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Characterising ISWI chromatin remodeler in Trypanosoma cruzi.Memorias do Instituto Oswaldo Cruz · 2020Article
- Rapid affinity purification of intracellular organelles using a twin strep tag.Journal of cell science · 2019Article
- Membrane phospholipid alteration causes chronic ER stress through early degradation of homeostatic ER-resident proteins.Scientific reports · 2019Article
- The chaperone ERp29 is required for tunneling nanotube formation by stabilizing MSec.The Journal of biological chemistry · 2019Article
- Identification of RNase-sensitive LINE-1 Ribonucleoprotein Interactions by Differential Affinity Immobilization.Bio-protocol · 2019Article
- The SEACIT complex is involved in the maintenance of vacuole-mitochondria contact sites and controls mitophagy.Cellular and molecular life sciences : CMLS · 2019Article
- The Galaxy Platform for Reproducible Affinity Proteomic Mass Spectrometry Data Analysis.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Tumor suppressor NPRL2 induces ROS production and DNA damage response.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with the co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, high specificity reagents to target and capture proteins of interest along with specifically associated proteins from cell extracts. Affinity capture coupled to mass spectrometry (MS)-based proteomic analyses has enabled the isolation and characterization of a wide range of endogenous protein complexes. Here, we outline effective procedures for the affinity capture of protein complexes, highlighting best practices and common pitfalls.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.