ArticleBioscience reports2015
Identification of signalling cascades involved in red blood cell shrinkage and vesiculation.
Article in Bioscience reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Bilberry Supplementation after Myocardial Infarction Decreases Microvesicles in Blood and Affects Endothelial Vesiculation.Molecular nutrition & food research · 2020Trial
- Vesiculation as potential novel pathogenic mechanism in autoimmune hemolytic anemia.Transfusion · 2025Article
- Red blood cell signaling is functionally conserved iniScience · 2024Article
- Identifying pleiotropic genes via the composite test amidst the complexity of polygenic traits.Briefings in bioinformatics · 2024Article
- Human red blood cells release microvesicles with distinct sizes and protein composition that alter neutrophil phagocytosis.Journal of extracellular biology · 2023Article
- Differences in selected blood parameters between brachycephalic and non-brachycephalic dogs.Frontiers in veterinary science · 2023Article
- Successful Treatment with Brigatinib after Alectinib-Induced Hemolytic Anemia in Patients with Metastatic Lung Adenocarcinoma-A Case Series.Current oncology (Toronto, Ont.) · 2022Article
- Band 3 Protein: An Effective Interrogation Tool of Storage Lesions in RBC Units.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2022Article
- The TRPV2 channel mediates Ca2+ influx and the Δ9-THC-dependent decrease in osmotic fragility in red blood cells.Haematologica · 2021Article
- Extracellular microvesicles/exosomes: discovery, disbelief, acceptance, and the future?Leukemia · 2020Review
- Microvesicle Formation Induced by Oxidative Stress in Human Erythrocytes.Antioxidants (Basel, Switzerland) · 2020Article
- Cellular microdomains for nitric oxide signaling in endothelium and red blood cells.Nitric oxide : biology and chemistry · 2020Review
- Reticulocyte and red blood cell deformation triggers specific phosphorylation events.Blood advances · 2019Article
- Uridine adenosine tetraphosphate and purinergic signaling in cardiovascular system: An update.Pharmacological research · 2019Review
- Glycophorin-C sialylation regulates Lu/BCAM adhesive capacity during erythrocyte aging.Blood advances · 2018Article
- Sickle Cell Disease: Advances in Treatment.Ochsner journal · 2018Review
- Erythrocyte Aging, Protection via Vesiculation: An Analysis Methodology via Oscillatory Flow.Frontiers in physiology · 2018Article
- Red Blood Cell Homeostasis: Mechanisms and Effects of Microvesicle Generation in Health and Disease.Frontiers in physiology · 2018Review
- Squeezing for Life - Properties of Red Blood Cell Deformability.Frontiers in physiology · 2018Review
- The Shape Shifting Story of Reticulocyte Maturation.Frontiers in physiology · 2018Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Even though red blood cell (RBC) vesiculation is a well-documented phenomenon, notably in the context of RBC aging and blood transfusion, the exact signalling pathways and kinases involved in this process remain largely unknown. We have established a screening method for RBC vesicle shedding using the Ca(2+) ionophore ionomycin which is a rapid and efficient method to promote vesiculation. In order to identify novel pathways stimulating vesiculation in RBC, we screened two libraries: the Library of Pharmacologically Active Compounds (LOPAC) and the Selleckchem Kinase Inhibitor Library for their effects on RBC from healthy donors. We investigated compounds triggering vesiculation and compounds inhibiting vesiculation induced by ionomycin. We identified 12 LOPAC compounds, nine kinase inhibitors and one kinase activator which induced RBC shrinkage and vesiculation. Thus, we discovered several novel pathways involved in vesiculation including G protein-coupled receptor (GPCR) signalling, the phosphoinositide 3-kinase (PI3K)-Akt (protein kinase B) pathway, the Jak-STAT (Janus kinase-signal transducer and activator of transcription) pathway and the Raf-MEK (mitogen-activated protein kinase kinase)-ERK (extracellular signal-regulated kinase) pathway. Moreover, we demonstrated a link between casein kinase 2 (CK2) and RBC shrinkage via regulation of the Gardos channel activity. In addition, our data showed that inhibition of several kinases with unknown functions in mature RBC, including Alk (anaplastic lymphoma kinase) kinase and vascular endothelial growth factor receptor 2 (VEGFR-2), induced RBC shrinkage and vesiculation.
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