ArticleThe ISME journal2015
Higher diversity and abundance of denitrifying microorganisms in environments than considered previously.
Article in The ISME journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
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- Genetic Potential for N₂O Metabolism in Tree Tissues: Insights From Nitrogen Cycling Gene Prevalence and nosZ Diversity Across Tree Species.Microbial ecology · 2026Article
- The hidden potential of archaea in carbon and nitrogen cycling in agricultural soils: a review.Frontiers in microbiology · 2026Review
- Interactions of microplastics with pesticides and anthelminthics mediate undesirable effects on microbial nitrogen cycling in agricultural soils.FEMS microbiology ecology · 2025Article
- The Interaction Among Rhizosphere Soil Nutrients, Metabolites, and Microbes Determines the Productivity of Perennial Cultivated Grassland in Qinghai-Tibet Plateau.Ecology and evolution · 2025Article
- Roles of coreFrontiers in plant science · 2025Article
- Metagenomic evidence clarifies the texture-dependent cascading effects of organic degradation on soil hypoxia and NFrontiers in microbiology · 2025Article
- Novel Stepped Combined Constructed Wetland For Surface Water Removal: Enhancing the Performance and Responses of Microbial Communities.ACS omega · 2024Article
- Analysis of Microbial Diversity Dominating Nitrite Enzymatic Degradation and Acidic Degradation in the Fermentation Broth of Northeast Sauerkraut.Foods (Basel, Switzerland) · 2024Article
- Enhanced salt tolerance inMicrobiology spectrum · 2024Article
- Soils of two Antarctic Dry Valleys exhibit unique microbial community structures in response to similar environmental disturbances.Environmental microbiome · 2024Article
- Inoculation withMicrobiology spectrum · 2024Article
- Comparative analyses on nitrogen removal microbes and functional genes within anaerobic-anoxic-oxic and deoxidation ditch sewage-treating processes in Wuhan and Xi'an cities, China.Frontiers in microbiology · 2024Article
- Phylogenetics and environmental distribution of nitric oxide-forming nitrite reductases reveal their distinct functional and ecological roles.ISME communications · 2024Article
- Denitrification genotypes of endospore-formingISME communications · 2024Article
- Article
- Partitioning of the denitrification pathway and other nitrite metabolisms within global oxygen deficient zones.ISME communications · 2023Article
- Potential relevance between soybean nitrogen uptake and rhizosphere prokaryotic communities under waterlogging stress.ISME communications · 2023Article
- Denitrification in hypersaline and coastal environments.FEMS microbiology letters · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Denitrification is an important process in the global nitrogen cycle. The genes encoding NirK and NirS (nirK and nirS), which catalyze the reduction of nitrite to nitric oxide, have been used as marker genes to study the ecological behavior of denitrifiers in environments. However, conventional polymerase chain reaction (PCR) primers can only detect a limited range of the phylogenetically diverse nirK and nirS. Thus, we developed new PCR primers covering the diverse nirK and nirS. Clone library and qPCR analysis using the primers showed that nirK and nirS in terrestrial environments are more phylogenetically diverse and 2-6 times more abundant than those revealed with the conventional primers. RNA- and culture-based analyses using a cropland soil also suggested that microorganisms with previously unconsidered nirK or nirS are responsible for denitrification in the soil. PCR techniques still have a greater capacity for the deep analysis of target genes than PCR-independent methods including metagenome analysis, although efforts are needed to minimize the PCR biases. The methodology and the insights obtained here should allow us to achieve a more precise understanding of the ecological behavior of denitrifiers and facilitate more precise estimate of denitrification in environments.
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