ArticlePeerJ2015
Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR.
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Development and multi-platform validation of a pan-genomic-driven PMA-qPCR method for the precise quantification of viableFrontiers in microbiology · 2026Article
- Antimicrobial Sub-MIC induces Staphylococcus aureus biofilm formation without affecting the bacterial count.BMC infectious diseases · 2024Article
- Dynamic immobilization of bacterial cells on biofilm in a polyester nonwoven chemostat.Bioresources and bioprocessing · 2024Article
- Evaluation of novel compounds as anti-bacterial or anti-virulence agents.Frontiers in cellular and infection microbiology · 2024Review
- Advances in the detection technology of vegetable soil borne fungi and bacteria.Frontiers in microbiology · 2024Review
- Practical application of PMA-qPCR assay for determination of viable cells of inter-species biofilm ofBiology methods & protocols · 2024Article
- An Improved Method for Quantification of ViableMicroorganisms · 2022Article
- Detection ofFrontiers in microbiology · 2022Article
- Dissecting Individual Interactions between Pathogenic and Commensal Bacteria within a Multispecies Gut Microbial Community.mSphere · 2021Article
- How to Evaluate Non-Growing Cells-Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Genetic determinants ofBiofilm · 2020Article
- Activity of Antibiotics against Pseudomonas aeruginosa in anAntimicrobial agents and chemotherapy · 2020Article
- RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms.Scientific reports · 2019Article
- Article
- Activity of Antibiotics against Staphylococcus aureus in anAntimicrobial agents and chemotherapy · 2019Article
- Influence of the lung microbiome on antibiotic susceptibility of cystic fibrosis pathogens.European respiratory review : an official journal of the European Respiratory Society · 2019Review
- Propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR) assay for rapid detection of viable and viable but non-culturable (VBNC)Journal of environmental health science & engineering · 2019Article
- Molecular viability testing of viable but non-culturable bacteria induced by antibiotic exposure.Microbial biotechnology · 2018Article
- Quantitative assessment of individual populations within polymicrobial biofilms.Scientific reports · 2018Article
- Community Composition Determines Activity of Antibiotics against Multispecies Biofilms.Antimicrobial agents and chemotherapy · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.