ArticleFrontiers in microbiology2015
Involvement of glycolysis/gluconeogenesis and signaling regulatory pathways in Saccharomyces cerevisiae biofilms during fermentation.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
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- Comparative fermentation performance of biofilm and planktonic Saccharomyces cerevisiae under standard and high-carbon media.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Pyruvate carboxylase is critical for biofilm formation in Mycobacterium tuberculosis.NPJ biofilms and microbiomes · 2026Article
- Galactooligosaccharides Based on β-Galactosidase-Catalyzed Synthesis: Function, Biosynthesis and Optimization Strategy.Foods (Basel, Switzerland) · 2026Review
- Transcriptional Response of Durum Wheat During Interaction withInternational journal of molecular sciences · 2026Article
- Study on the Plateau Adaptive Synergistic Mechanism of Rumen Microbiome-Metabolome-Resistome in Tibetan Sheep.Microorganisms · 2025Article
- Biofilm-based biocatalysis for β-cyclodextrin production by the surface-display of β-cyclodextrin glycosyltransferase in Bacillus subtilis.Scientific reports · 2024Article
- Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase inInternational journal of molecular sciences · 2023Article
- Aminotransferase SsAro8 Regulates Tryptophan Metabolism Essential for Filamentous Growth of Sugarcane Smut FungusMicrobiology spectrum · 2022Article
- Cell Cycle Progression Influences Biofilm Formation in Saccharomyces cerevisiae 1308.Microbiology spectrum · 2022Article
- Microbial biofilm: formation, architecture, antibiotic resistance, and control strategies.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Genetic analysis of electroactive biofilms.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Mechanism Underlying the Role of LuxR Family Transcriptional Regulator abaR in Biofilm Formation by Acinetobacter baumannii.Current microbiology · 2021Article
- Effect of quorum-sensing molecule 2-phenylethanol and ARO genes on Saccharomyces cerevisiae biofilm.Applied microbiology and biotechnology · 2021Article
- Approaches for Mitigating Microbial Biofilm-Related Drug Resistance: A Focus on Micro- and Nanotechnologies.Molecules (Basel, Switzerland) · 2021Review
- Staphylococcus aureus induces an itaconate-dominated immunometabolic response that drives biofilm formation.Nature communications · 2021Article
- Biofilm-Related, Time-Series Transcriptome and Genome Sequencing in Xylanase-ProducingACS omega · 2020Article
- Comparative proteomic and genomic analyses of Brucella abortus biofilm and planktonic cells.Molecular medicine reports · 2020Article
- CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata.Scientific reports · 2019Article
- Feasibility Study on Long-Term Continuous Ethanol Production from Cassava Supernatant by Immobilized Yeast Cells in Packed Bed Reactor.Journal of microbiology and biotechnology · 2019Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compared to free (free-living) cells, biofilm cells show increased resistance and stability to high-pressure fermentation conditions, although the reasons underlying these phenomena remain unclear. Here, we investigated biofilm formation with immobilized Saccharomyces cerevisiae cells grown on fiber surfaces during the process of ethanol fermentation. The development of biofilm colonies was visualized by fluorescent labeling and confocal microscopy. RNA from yeast cells at three different biofilm development periods was extracted and sequenced by high-throughput sequencing. We quantitated gene expression differences between biofilm cells and free cells and found that 2098, 1556, and 927 genes were significantly differentially expressed, respectively. We also validated the expression of previously reported genes and identified novel genes and pathways under the control of this system. Statistical analysis revealed that biofilm genes show significant gene expression changes principally in the initial period of biofilm formation compared to later periods. Carbohydrate metabolism, amino acid metabolism, signal transduction, and oxidoreductase activity were needed for biofilm formation. In contrast to previous findings, we observed some differential expression performances of FLO family genes, indicating that cell aggregation in our immobilized fermentation system was possibly independent of flocculation. Cyclic AMP-protein kinase A and mitogen-activated protein kinase pathways regulated signal transduction pathways during yeast biofilm formation. We found that carbohydrate metabolism, especially glycolysis/gluconeogenesis, played a key role in the development of S. cerevisiae biofilms. This work provides an important dataset for future studies aimed at gaining insight into the regulatory mechanisms of immobilized cells in biofilms, as well as for optimizing bioprocessing applications with S. cerevisiae.
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