ArticleStem cell reports2015
Direct reprogramming of human bone marrow stromal cells into functional renal cells using cell-free extracts.
Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Ways and Means of Cellular Reconditioning for Kidney Regeneration.American journal of nephrology · 2022Review
- Article
- Direct cell-fate conversion of somatic cells: Toward regenerative medicine and industries.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2020Review
- New Trends in Regenerative Medicine: Reprogramming and Reconditioning.Journal of the American Society of Nephrology : JASN · 2019Article
- A validated collection of mouse monoclonal antibodies to human glycosyltransferases functioning in mucin-type O-glycosylation.Glycobiology · 2019Article
- Engineering renal epithelial cells: programming and directed differentiation towards glomerular podocyte's progenitor and mature podocyte.American journal of translational research · 2019Review
- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Instructive Role of the Microenvironment in Preventing Renal Fibrosis.Stem cells translational medicine · 2017Article
- (Mesenchymal) Stem Cell-Based Therapy in Cisplatin-Induced Acute Kidney Injury Animal Model: Risk of Immunogenicity and Tumorigenicity.Stem cells international · 2017Review
- Direct reprogramming of fibroblasts into renal tubular epithelial cells by defined transcription factors.Nature cell biology · 2016Article
- Regenerating a kidney in a lymph node.Pediatric nephrology (Berlin, Germany) · 2016Review
- Adult stem cells as a tool for kidney regeneration.World journal of nephrology · 2016Review
- Mesenchymal Stromal Cells Epithelial Transition Induced by Renal Tubular Cells-Derived Extracellular Vesicles.PloS one · 2016Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
The application of cell-based therapies in regenerative medicine is gaining recognition. Here, we show that human bone marrow stromal cells (BMSCs), also known as bone-marrow-derived mesenchymal cells, can be reprogrammed into renal proximal tubular-like epithelial cells using cell-free extracts. Streptolysin-O-permeabilized BMSCs exposed to HK2-cell extracts underwent morphological changes-formation of "domes" and tubule-like structures-and acquired epithelial functional properties such as transepithelial-resistance, albumin-binding, and uptake and specific markers E-cadherin and aquaporin-1. Transmission electron microscopy revealed the presence of brush border microvilli and tight intercellular contacts. RNA sequencing showed tubular epithelial transcript abundance and revealed the upregulation of components of the EGFR pathway. Reprogrammed BMSCs integrated into self-forming kidney tissue and formed tubular structures. Reprogrammed BMSCs infused in immunodeficient mice with cisplatin-induced acute kidney injury engrafted into proximal tubuli, reduced renal injury and improved function. Thus, reprogrammed BMSCs are a promising cell resource for future cell therapy.
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Registered trials
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