ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2015
Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF-β3-Associated Chondrogenesis of Bone Marrow-Derived Mesenchymal Stem Cells for Articular Surface Regeneration.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
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- Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells.International journal of molecular sciences · 2022Review
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- Tryptophan and Kynurenine Enhances the Stemness and Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro and In Vivo.Materials (Basel, Switzerland) · 2021Article
- Naringin and bone marrow mesenchymal stem cells repair articular cartilage defects in rabbit knees through the transforming growth factor-β superfamily signaling pathway.Experimental and therapeutic medicine · 2020Article
- Enhancement of chondrogenic differentiation supplemented by a novel small compound for chondrocyte-based tissue engineering.Journal of experimental orthopaedics · 2020Article
- Determinants of stem cell lineage differentiation toward chondrogenesis versus adipogenesis.Cellular and molecular life sciences : CMLS · 2019Review
- Biomimetic mineralization using matrix vesicle nanofragments.Journal of biomedical materials research. Part A · 2019Article
- Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells.International journal of molecular sciences · 2019Article
- New Functions of Classical Compounds against Orofacial Inflammatory Lesions.Medicines (Basel, Switzerland) · 2018Review
- Impact of dexamethasone concentration on cartilage tissue formation from human synovial derived stem cells in vitro.Cytotechnology · 2018Article
- A Review of the Effectiveness and Side-Effects of Fluocinolone Acetonide 0.1% in the Treatment of Oral Mucosal Diseases.Acta stomatologica Croatica · 2017Review
- Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells.Molecular medicine reports · 2016Article
- Article
- The repair of full-thickness articular cartilage defect using intra-articular administration of N-acetyl-D-glucosamine in the rabbit knee: randomized controlled trial.Biomedical engineering online · 2015Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Articular cartilage repair remains a challenging problem. Based on a high-throughput screening and functional analysis, we found that fluocinolone acetonide (FA) in combination with transforming growth factor beta 3 (TGF-β3) strongly potentiated chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). In an in vivo cartilage defect model in knee joints of immunocompromised mice, transplantation of FA/TGF-β3-treated hBMSCs could completely repair the articular surface. Analysis of the intracellular pathways revealed that FA enhanced TGF-β3-induced phosphorylation of Smad2 and Smad3. Additionally, we performed a pathway array and found that FA activates the mTORC1/AKT pathway. Chemical inhibition of mTORC1 with rapamycin substantially suppressed FA effect, and inhibition of AKT completely repressed chondrogenesis of hBMSCs. Inhibition of glucocorticoid receptor with mifepristone also suppressed FA effect, suggesting that FA involves binding to the glucocorticoid receptor. Comparative analysis with other glucocorticoids (triamcinolone acetonide [TA] and dexamethasone [DEX]) revealed the unique ability of FA to repair articular cartilage surgical defects. Analysis of intracellular pathways showed that the mTORC1/AKT pathway and the glucocorticoid receptor was highly activated with FA and TA, but to a lesser extent with DEX. Collectively, these results show a unique ability of FA to enhance TGF-β3-associated chondrogenesis, and suggest that the FA/TGF-β3 combination may be used as major inducer of chondrogenesis in vitro. Additionally, FA/TGF-β3 could be potentially applied in a clinical setting to increase the efficiency of regenerative approaches based on chondrogenic differentiation of stem cells.
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