ArticleInternational journal of legal medicine2015
Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination.
Article in International journal of legal medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review.Frontiers in medicine · 2021Pooled it
- Expression of matrix metalloproteinase-9 (MMP-9) in human skin within 1 hour after injury through immunohistochemical staining: a pilot study.International journal of legal medicine · 2024Article
- Review
- Dating Skin Lesions of Forensic Interest by Immunohistochemistry and Immunofluorescence Techniques: A Scoping Literature Review.Diagnostics (Basel, Switzerland) · 2024Article
- Relationship between intrathrombotic appearance of HSP27 and HSP70 and thrombus ages in a murine model of deep vein thrombosis.Scientific reports · 2023Article
- Detection of multiple biomarkers associated with satellite cell fate in the contused skeletal muscle of rats for wound age estimation.International journal of legal medicine · 2023Article
- Macrophage polarity and wound age determination.Scientific reports · 2022Article
- Estimation of Early Postmortem Interval from Long Noncoding RNA Gene Expression in the Incised Cutaneous Wound: An Experimental Study.Biomedicines · 2022Article
- Analysis of the risk of traumatic brain injury and evaluation neurogranin and myelin basic protein as potential biomarkers of traumatic brain injury in postmortem examination.Forensic science, medicine, and pathology · 2022Article
- Bone Marrow-Derived Cells and Wound Age Estimation.Frontiers in medicine · 2022Review
- Longitudinal peripheral tissue RNA-Seq transcriptomic profiling, hyperalgesia, and wound healing in the rat plantar surgical incision model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Intrathrombotic appearances of AQP-1 and AQP-3 in relation to thrombus age in murine deep vein thrombosis model.International journal of legal medicine · 2021Article
- Wound Vitality in Decomposed Bodies: New Frontiers Through Immunohistochemistry.Frontiers in medicine · 2021Article
- The Role of miRNAs as New Molecular Biomarkers for Dating the Age of Wound Production: A Systematic Review.Frontiers in medicine · 2021Review
- Article
- Mini Review: Forensic Value of Aquaporines.Frontiers in medicine · 2021Review
- Vitality and wound-age estimation in forensic pathology: review and future prospects.Forensic sciences research · 2020Review
- Temporal expression of wound healing-related genes inform wound age estimation in rats after a skeletal muscle contusion: a multivariate statistical model analysis.International journal of legal medicine · 2020Article
- Decreased collagen type III synthesis in skin fibroblasts is associated with parastomal hernia following colostomy.International journal of molecular medicine · 2019Article
- CXCL1 and CXCR2 as potential markers for vital reactions in skin contusions.Forensic science, medicine, and pathology · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0-3 days (n = 16); II, 4-7 days (n = 11); III, 9-14 days (n = 16); and IV, 17-21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-2(+) macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-9(+) signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-9(+) macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9-12 days, MMP-2(+) cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-2(+) macrophages of >20 would indicate a wound age of 7-12 days. Additionally, 10 out of 12 wound specimens aged 9-12 days showed the MMP-2(+) cell number of >25, implying that MMP-2(+) cell number of >25 would indicate the wound age of 9-12 days. On the contrary, all wound samples aged 3-14 days except for only one sample had MMP-9(+) cell number of >30, indicating that MMP-9(+) cell number of >30 would indicate the wound age of 3-14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9.
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