ArticleJournal of fluorescence2015
New insights on the fluorescent emission spectra of Prodan and Laurdan.
Article in Journal of fluorescence, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
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- DODAB-ODN lipoplex structure is highly dependent on ODN concentration: A multitechnique experimental study.Biochemistry and biophysics reports · 2025Article
- Enhancement of enzymatic activity by biomolecular condensates through pH buffering.Nature communications · 2025Article
- Multifocal lipid membrane characterization by combination of DAS-deconvolution and anisotropy.Biophysical journal · 2024Article
- Multiplicity of solvent environments in lipid bilayer revealed by DAS deconvolution of twin probes: Comparative method of Laurdan and Prodan.Biophysical journal · 2023Article
- Investigation of the Membrane Fluidity Regulation of Fatty Acid Intracellular Distribution by Fluorescence Lifetime Imaging of Novel Polarity Sensitive Fluorescent Derivatives.International journal of molecular sciences · 2021Article
- Interaction of synthetic antimicrobial peptides of the Hylin a1 family with models of eukaryotic structures: Zwitterionic membranes and DNA.Biochemistry and biophysics reports · 2020Article
- Ultrafast Formation of the Charge Transfer State of Prodan Reveals Unique Aspects of the Chromophore Environment.The journal of physical chemistry. B · 2020Article
- Fluorescence Quenching of Carbonyl-Twisted 5ACS omega · 2019Article
- Solvatochromic Modeling of Laurdan for Multiple Polarity Analysis of Dihydrosphingomyelin Bilayer.Biophysical journal · 2019Article
- Article
- DPPC Bilayers in Solutions of High Sucrose Content.Biophysical journal · 2018Article
- Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane.Journal of physics D: Applied physics · 2017Article
- Electrooptical Absorption Measurements (EOAM) Testify Existence of two Conformers of Prodan and Laurdan with Different Dipole Moments in Equilibrium Ground and Franck-Condon Excited State.Journal of fluorescence · 2016Article
- Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular membranes.Scientific reports · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prodan and Laurdan are fluorescent probes largely used in biological systems. They were synthetized to be sensitive to the environment polarity, and their fluorescent emission spectrum shifts around 120 nm, from cyclohexane to water. Although accepted that their emission spectrum is composed by two emission bands, the origin of these two bands is still a matter of discussion. Here we analyze the fluorescent spectra of Prodan and Laurdan in solvents of different polarities, both by decomposing the spectrum into two Gaussian bands and by computing the Decay Associated Spectra (DAS), the latter with time resolved fluorescence. Our data show that the intensity of the lower energy emission band of Prodan and Laurdan (attributed, in the literature, to the decay of a solvent relaxed state) is higher in cyclohexane than in water, showing a decrease as the polarity of the medium increases. Moreover, in all solvents studied here, the balance between the two emission bands is not dependent on the temperature, strongly suggesting two independent excited states. Both bands were found to display a red shift as the medium polarity increases. We propose here a new interpretation for the two emission bands of Prodan and Laurdan in homogeneous solvents: they would be related to the emission of two independent states, and not to a pair of non-relaxed and solvent relaxed states.
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