ArticleJournal of cellular and molecular medicine2015
Gene expression modulation in TGF-β3-mediated rabbit bone marrow stem cells using electrospun scaffolds of various stiffness.
Article in Journal of cellular and molecular medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Engineering Intervertebral Disc Regeneration: Biomaterials, Cell Sources and Animal Models.Cell proliferation · 2025Review
- Administration of mRNA-Nanomedicine-Augmented Calvarial Defect Healing via Endochondral Ossification.Pharmaceutics · 2023Article
- Polymeric and Composite Carriers of Protein and Non-Protein Biomolecules for Application in Bone Tissue Engineering.Materials (Basel, Switzerland) · 2023Review
- Core-shell oxygen-releasing fibers for annulus fibrosus repair in the intervertebral disc of rats.Materials today. Bio · 2023Article
- Polyphenols-loaded electrospun nanofibers in bone tissue engineering and regeneration.Biomaterials research · 2021Review
- Putting the Pieces in Place: Mobilizing Cellular Players to Improve Annulus Fibrosus Repair.Tissue engineering. Part B, Reviews · 2021Review
- Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds.Journal of orthopaedic translation · 2021Article
- Genipin-crosslinked decellularized annulus fibrosus hydrogels induces tissue-specific differentiation of bone mesenchymal stem cells and intervertebral disc regeneration.Journal of tissue engineering and regenerative medicine · 2020Article
- Biomaterials-Induced Stem Cells Specific Differentiation Into Intervertebral Disc Lineage Cells.Frontiers in bioengineering and biotechnology · 2020Review
- Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration.RSC advances · 2019Article
- Strategies for Annulus Fibrosus Regeneration: From Biological Therapies to Tissue Engineering.Frontiers in bioengineering and biotechnology · 2018Review
- Equiaxial Strain Modulates Adipose-derived Stem Cell Differentiation within 3D Biphasic Scaffolds towards Annulus Fibrosus.Scientific reports · 2017Article
- Effect of matrix stiffness on osteoblast functionalization.Cell proliferation · 2017Article
- Current strategies for treatment of intervertebral disc degeneration: substitution and regeneration possibilities.Biomaterials research · 2017Review
- Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells.Molecular medicine reports · 2016Article
- [Progress and challenges in tissue engineering of intervertebral disc annulus fibrosus].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2016Review
- Effect of scaffold elasticity on the gene expression of annulus fibrosus-derived stem cells.Data in brief · 2015Article
- The effect of the fibre orientation of electrospun scaffolds on the matrix production of rabbit annulus fibrosus-derived stem cells.Bone research · 2015Article
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue engineering has recently evolved into a promising approach for annulus fibrosus (AF) regeneration. However, selection of an ideal cell source, which can be readily differentiated into AF cells of various regions, remains challenging because of the heterogeneity of AF tissue. In this study, we set out to explore the feasibility of using transforming growth factor-β3-mediated bone marrow stem cells (tBMSCs) for AF tissue engineering. Since the differentiation of stem cells significantly relies on the stiffness of substrate, we fabricated nanofibrous scaffolds from a series of biodegradable poly(ether carbonate urethane)-urea (PECUU) materials whose elastic modulus approximated that of native AF tissue. We cultured tBMSCs on PECUU scaffolds and compared their gene expression profile to AF-derived stem cells (AFSCs), the newly identified AF tissue-specific stem cells. As predicted, the expression of collagen-I in both tBMSCs and AFSCs increased with scaffold stiffness, whereas the expression of collagen-II and aggrecan genes showed an opposite trend. Interestingly, the expression of collagen-I, collagen-II and aggrecan genes in tBMSCs on PECUU scaffolds were consistently higher than those in AFSCs regardless of scaffold stiffness. In addition, the cell traction forces (CTFs) of both tBMSCs and AFSCs gradually decreased with scaffold stiffness, which is similar to the CTF change of cells from inner to outer regions of native AF tissue. Together, findings from this study indicate that tBMSCs had strong tendency to differentiate into various types of AF cells and presented gene expression profiles similar to AFSCs, thereby establishing a rationale for the use of tBMSCs in AF tissue engineering.
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