ArticleMolecular cell2015
Two distinct DNA binding modes guide dual roles of a CRISPR-Cas protein complex.
Article in Molecular cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 103 citations in OpenAlex.
- Single molecule technique unveils the role of electrostatic interactions in ssDNA-gp32 molecular complex stability.RSC advances · 2024Article
- Widespread CRISPR-derived RNA regulatory elements in CRISPR-Cas systems.Nucleic acids research · 2023Article
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- Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3.Nature communications · 2022Article
- CRISPR-Cas9 bends and twists DNA to read its sequence.Nature structural & molecular biology · 2022Article
- Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer.Nucleic acids research · 2021Article
- Article
- Extracting Transition Rates in Particle Tracking Using Analytical Diffusion Distribution Analysis.Biophysical journal · 2020Article
- DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features.Nucleic acids research · 2020Article
- Cas3/I-C mediated target DNA recognition and cleavage during CRISPR interference are independent of the composition and architecture of Cascade surveillance complex.Nucleic acids research · 2020Article
- Cas9 Cleavage of Viral Genomes Primes the Acquisition of New Immunological Memories.Cell host & microbe · 2019Article
- A seed motif for target RNA capture enables efficient immune defence by a type III-B CRISPR-Cas system.RNA biology · 2019Article
- Article
- Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas.Molecular cell · 2019Article
- Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation.Molecular microbiology · 2019Article
- Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry.Molecular cell · 2019Article
- Mechanisms of Type I-E and I-F CRISPR-Cas Systems inEcoSal Plus · 2019Review
- Dynamics of Cas10 Govern Discrimination between Self and Non-self in Type III CRISPR-Cas Immunity.Molecular cell · 2019Article
- Molecular mechanisms of CRISPR-Cas spacer acquisition.Nature reviews. Microbiology · 2019Review
- Role of nucleotide identity in effective CRISPR target escape mutations.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Small RNA-guided protein complexes play an essential role in CRISPR-mediated immunity in prokaryotes. While these complexes initiate interference by flagging cognate invader DNA for destruction, recent evidence has implicated their involvement in new CRISPR memory formation, called priming, against mutated invader sequences. The mechanism by which the target recognition complex mediates these disparate responses-interference and priming-remains poorly understood. Using single-molecule FRET, we visualize how bona fide and mutated targets are differentially probed by E. coli Cascade. We observe that the recognition of bona fide targets is an ordered process that is tightly controlled for high fidelity. Mutated targets are recognized with low fidelity, which is featured by short-lived and PAM- and seed-independent binding by any segment of the crRNA. These dual roles of Cascade in immunity with distinct fidelities underpin CRISPR-Cas robustness, allowing for efficient degradation of bona fide targets and priming of mutated DNA targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.