ArticlePloS one2015
In silico analysis of usher encoding genes in Klebsiella pneumoniae and characterization of their role in adhesion and colonization.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
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- Targeting of Bacteria Using Amylase-Degradable, Copper-Loaded Starch Nanoparticles.Antibiotics (Basel, Switzerland) · 2026Article
- A novelVirulence · 2025Article
- Biofilm and Outer Membrane Vesicle Formation in ESKAPE Gram-Negative Bacteria: A Comprehensive Review.International journal of molecular sciences · 2025Review
- How Klebsiella pneumoniae controls its virulence.PLoS pathogens · 2025Review
- High-fat and low-fiber diet elevates the gut resistome: a comparative metagenomic study.NPJ biofilms and microbiomes · 2025Article
- Deciphering the gastrointestinal carriage ofInfection and immunity · 2024Review
- In silico analysis of Ffp1, an ancestral Porphyromonas spp. fimbrillin, shows differences with Fim and Mfa.Access microbiology · 2024Article
- Article
- Latent evolution of biofilm formation depends on life-history and genetic background.NPJ biofilms and microbiomes · 2023Article
- In-Silico Analysis Highlights the Existence in Members ofMicroorganisms · 2023Article
- Regulation of biofilm formation inFrontiers in microbiology · 2023Review
- Klebsiella pneumoniae l-Fucose Metabolism Promotes Gastrointestinal Colonization and Modulates Its Virulence Determinants.Infection and immunity · 2022Article
- Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization.Scientific reports · 2022Article
- Characteristics of community-acquired carbapenem-resistant Enterobacterales.The Journal of antimicrobial chemotherapy · 2022Article
- Combined comparative genomics and clinical modeling reveals plasmid-encoded genes are independently associated with Klebsiella infection.Nature communications · 2022Article
- Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-ResistantInternational journal of molecular sciences · 2022Article
- Review
- FromMicroorganisms · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chaperone/usher (CU) assembly pathway is used by a wide range of Enterobacteriaceae to assemble adhesive surface structures called pili or fimbriae that play a role in bacteria-host cell interactions. In silico analysis revealed that the genome of Klebsiella pneumoniae LM21 harbors eight chromosomal CU loci belonging to γκп and ϭ clusters. Of these, only two correspond to previously described operons, namely type 1 and type 3-encoding operons. Isogenic usher deletion mutants of K. pneumoniae LM21 were constructed for each locus and their role in adhesion to animal (Intestine 407) and plant (Arabidopsis thaliana) cells, biofilm formation and murine intestinal colonization was investigated. Type 3 pili usher deleted mutant was impaired in all assays, whereas type 1 pili usher deleted mutant only showed attenuation in adhesion to plant cells and in intestinal colonization. The LM21ΔkpjC mutant was impaired in its capacity to adhere to Arabidopsis cells and to colonize the murine intestine, either alone or in co-inoculation experiments. Deletion of LM21kpgC induced a significant decrease in biofilm formation, in adhesion to animal cells and in colonization of the mice intestine. The LM21∆kpaC and LM21∆kpeC mutants were only attenuated in biofilm formation and the adhesion abilities to Arabidopsis cells, respectively. No clear in vitro or in vivo effect was observed for LM21∆kpbC and LM21∆kpdC mutants. The multiplicity of CU loci in K. pneumoniae genome and their specific adhesion pattern probably reflect the ability of the bacteria to adhere to different substrates in its diverse ecological niches.
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