ArticlePloS one2015
Distinct human stem cell populations in small and large intestine.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Adult Bovine-Derived Small and Large Intestinal Organoids:Journal of tissue engineering and regenerative medicine · 2023Article
- IgGFc-binding protein and MUC2 mucin produced by colonic goblet-like cells spatially interact non-covalently and regulate wound healing.Frontiers in immunology · 2023Article
- The World of Organoids: Gastrointestinal Disease Modelling in the Age of 3R and One Health with Specific Relevance to Dogs and Cats.Animals : an open access journal from MDPI · 2022Review
- Generation of human colon organoids from healthy and inflammatory bowel disease mucosa.PloS one · 2022Article
- Drivers of transcriptional variance in human intestinal epithelial organoids.Physiological genomics · 2021Article
- RAD51AP1 Loss Attenuates Colorectal Cancer Stem Cell Renewal and Sensitizes to Chemotherapy.Molecular cancer research : MCR · 2021Article
- Organoid-based Models to Study the Role of Host-microbiota Interactions in IBD.Journal of Crohn's & colitis · 2021Review
- Zebrafish harbor diverse intestinal macrophage populations including a subset intimately associated with enteric neural processes.iScience · 2021Article
- Corticosteroid enhances epithelial barrier function in intestinal organoids derived from patients with Crohn's disease.Journal of molecular medicine (Berlin, Germany) · 2021Article
- Vitamin DStem cell research & therapy · 2021Article
- The Role of Organoids as a Novel Platform for Modeling of Inflammatory Bowel Disease.Frontiers in pediatrics · 2021Review
- Comparative Study of Organoids from Patient-Derived Normal and Tumor Colon and Rectal Tissue.Cancers · 2020Article
- DNA Methylation Analysis Validates Organoids as a Viable Model for Studying Human Intestinal Aging.Cellular and molecular gastroenterology and hepatology · 2020Article
- Intestinal enteroids/organoids: A novel platform for drug discovery in inflammatory bowel diseases.World journal of gastroenterology · 2019Review
- Establishment and Characterization of 5-Fluorouracil-Resistant Human Colorectal Cancer Stem-Like Cells: Tumor Dynamics under Selection Pressure.International journal of molecular sciences · 2019Article
- Article
- Intestinal Organoids as a Novel Complementary Model to Dissect Inflammatory Bowel Disease.Stem cells international · 2019Review
- Potential Use of Human Stem Cell-Derived Intestinal Organoids to Study Inflammatory Bowel Diseases.Inflammatory bowel diseases · 2018Review
- Article
- Gut Microbial Influences on the Mammalian Intestinal Stem Cell Niche.Stem cells international · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The intestine is composed of an epithelial layer containing rapidly proliferating cells that mature into two regions, the small and the large intestine. Although previous studies have identified stem cells as the cell-of-origin for intestinal epithelial cells, no studies have directly compared stem cells derived from these anatomically distinct regions. Here, we examine intrinsic differences between primary epithelial cells isolated from human fetal small and large intestine, after in vitro expansion, using the Wnt agonist R-spondin 2. We utilized flow cytometry, fluorescence-activated cell sorting, gene expression analysis and a three-dimensional in vitro differentiation assay to characterize their stem cell properties. We identified stem cell markers that separate subpopulations of colony-forming cells in the small and large intestine and revealed important differences in differentiation, proliferation and disease pathways using gene expression analysis. Single cells from small and large intestine cultures formed organoids that reflect the distinct cellular hierarchy found in vivo and respond differently to identical exogenous cues. Our characterization identified numerous differences between small and large intestine epithelial stem cells suggesting possible connections to intestinal disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.