ArticleSensors (Basel, Switzerland)2015
A portable automatic endpoint detection system for amplicons of loop mediated isothermal amplification on microfluidic compact disk platform.
Article in Sensors (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Enhanced Sweat Biosensing with Thread-Embedded Microfluidic Devices.Medical science monitor : international medical journal of experimental and clinical research · 2024Article
- Low-Cost Arduino Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) for Sensitive Nucleic Acid Detection.Biosensors · 2024Article
- Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip.Mikrochimica acta · 2022Article
- Three-Way Junction-Induced Isothermal Amplification with High Signal-to-Background Ratio for Detection of Pathogenic Bacteria.Sensors (Basel, Switzerland) · 2021Article
- Fully Automated Lab-On-A-Disc Platform for Loop-Mediated Isothermal Amplification Using Micro-Carbon-Activated Cell Lysis.Sensors (Basel, Switzerland) · 2020Article
- Microfluidic-Based Approaches for Foodborne Pathogen Detection.Microorganisms · 2019Review
- Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform.Sensors (Basel, Switzerland) · 2019Article
- Loop-Mediated Isothermal Amplification for Salmonella Detection in Food and Feed: Current Applications and Future Directions.Foodborne pathogens and disease · 2018Review
- A Microfluidic Lab-on-a-Disc (LOD) for Antioxidant Activities of Plant Extracts.Micromachines · 2018Article
- Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.Journal of applied microbiology · 2018Review
- Lab-on-a-Disc Platform for Automated Chemical Cell Lysis.Sensors (Basel, Switzerland) · 2018Article
- Rapid Waterborne Pathogen Detection with Mobile Electronics.Sensors (Basel, Switzerland) · 2017Article
- A Review of Biomedical Centrifugal Microfluidic Platforms.Micromachines · 2016Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, many improvements have been made in foodborne pathogen detection methods to reduce the impact of food contamination. Several rapid methods have been developed with biosensor devices to improve the way of performing pathogen detection. This paper presents an automated endpoint detection system for amplicons generated by loop mediated isothermal amplification (LAMP) on a microfluidic compact disk platform. The developed detection system utilizes a monochromatic ultraviolet (UV) emitter for excitation of fluorescent labeled LAMP amplicons and a color sensor to detect the emitted florescence from target. Then it processes the sensor output and displays the detection results on liquid crystal display (LCD). The sensitivity test has been performed with detection limit up to 2.5 × 10(-3) ng/µL with different DNA concentrations of Salmonella bacteria. This system allows a rapid and automatic endpoint detection which could lead to the development of a point-of-care diagnosis device for foodborne pathogens detection in a resource-limited environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.