ArticlePloS one2015
Nepenthesin protease activity indicates digestive fluid dynamics in carnivorous nepenthes plants.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 60 citations in OpenAlex.
- A Native Nepenthesin Reactor for Improved Proteolytic Digestion of Intrinsically Disordered Proteins in Proteomics Workflows.Chembiochem : a European journal of chemical biology · 2026Article
- Transcriptomic prey-capture responses in convergently evolved carnivorous pitcher plants.The New phytologist · 2026Article
- The Localization of Cell Wall Components in the Whole-Mount ImmunolabeledInternational journal of molecular sciences · 2025Article
- How the diversity in digestion in carnivorous plants may have evolved.The New phytologist · 2025Review
- Carnivorous plants can decompose the polyesters poly(ethylene terephthalate) and poly(butylene adipate terephthalate).Scientific reports · 2025Article
- Recruitment of Sugar Transport and Scent Volatile Genes for Prey Attraction in the Nectar Spoon of Heliamphora tatei.Evolution & development · 2025Article
- Complex transcription regulation of acidic chitinase suggests fine-tuning of digestive processes in Drosera binata.Planta · 2025Article
- An acidophilic fungus promotes prey digestion in a carnivorous plant.Nature microbiology · 2024Article
- Is the co-option of jasmonate signalling for botanical carnivory a universal trait for all carnivorous plants?Journal of experimental botany · 2024Article
- Do Reactive Oxygen and Nitrogen Species Have a Similar Effect on Digestive Processes in CarnivorousBiology · 2023Review
- Omics Approaches in Uncovering Molecular Evolution and Physiology of Botanical Carnivory.Plants (Basel, Switzerland) · 2023Review
- The digestive systems of carnivorous plants.Plant physiology · 2022Review
- Article
- The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous PlantBiomolecules · 2020Article
- Metabolomics Analysis Reveals Tissue-Specific Metabolite Compositions in Leaf Blade and Traps of CarnivorousInternational journal of molecular sciences · 2020Article
- Molecular Properties and New Potentials of Plant Nepenthesins.Plants (Basel, Switzerland) · 2020Review
- Transcriptome-wide shift from photosynthesis and energy metabolism upon endogenous fluid protein depletion in young Nepenthes ampullaria pitchers.Scientific reports · 2020Article
- Tropical pitcher plants (Nepenthes) act as ecological filters by altering properties of their fluid microenvironments.Scientific reports · 2020Article
- Transcriptomic and Proteomic Analyses ofFrontiers in plant science · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carnivorous plants use different morphological features to attract, trap and digest prey, mainly insects. Plants from the genus Nepenthes possess specialized leaves called pitchers that function as pitfall-traps. These pitchers are filled with a digestive fluid that is generated by the plants themselves. In order to digest caught prey in their pitchers, Nepenthes plants produce various hydrolytic enzymes including aspartic proteases, nepenthesins (Nep). Knowledge about the generation and induction of these proteases is limited. Here, by employing a FRET (fluorescent resonance energy transfer)-based technique that uses a synthetic fluorescent substrate an easy and rapid detection of protease activities in the digestive fluids of various Nepenthes species was feasible. Biochemical studies and the heterologously expressed Nep II from Nepenthes mirabilis proved that the proteolytic activity relied on aspartic proteases, however an acid-mediated auto-activation mechanism was necessary. Employing the FRET-based approach, the induction and dynamics of nepenthesin in the digestive pitcher fluid of various Nepenthes plants could be studied directly with insect (Drosophila melanogaster) prey or plant material. Moreover, we observed that proteolytic activity was induced by the phytohormone jasmonic acid but not by salicylic acid suggesting that jasmonate-dependent signaling pathways are involved in plant carnivory.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.