ArticleThe EMBO journal2015
Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
Article in The EMBO journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 137 citations in OpenAlex.
- Characterization of the metabolome and proteome of stem cell-derived human primordial germ cell-like cells: a multi-omics approach.Biology open · 2026Article
- Generation of human primordial germ cell-like cells from cumulus and blood cell-derived isogenic human induced pluripotent stem cells†.Biology of reproduction · 2026Article
- Single-cell transcriptomics of organoids reveals transcriptional control of germline stem cell fate by an E2F1-TFAP2C-SOX17 positive-feedback loop in Turner syndrome.Cell & bioscience · 2025Article
- Stem cell-derived gametes: what to expect when expecting their clinical introduction.Human reproduction (Oxford, England) · 2025Review
- A Polyomavirus-Positive Merkel Cell Carcinoma Mouse Model Supports a Unified Origin for Somatic and Germ Cell Cancers.Cancers · 2025Article
- Application of induced pluripotent stem cells in the conservation of endangered animals.Stem cell research & therapy · 2025Review
- Evolving Lessons on Metazoan Primordial Germ Cells in Diversity and Development.Molecular reproduction and development · 2025Review
- ImprovingJournal of biomedical research · 2025Article
- The emergence of human primordial germ cell-like cells in stem cell-derived gastruloids.Science advances · 2025Article
- The establishment and regulation of human germ cell lineage.Stem cell research & therapy · 2025Review
- Generating human primordial germ cell-like cells from pluripotent stem cells: a scoping review ofHuman reproduction open · 2025Review
- Research advances in the construction of stem cell-derived ovarian organoids.Stem cell research & therapy · 2024Review
- Generation of marmoset primordial germ cell-like cells under chemically defined conditions.Life science alliance · 2024Article
- The many dimensions of germline competence.Current opinion in cell biology · 2023Review
- Expanded Potential Stem Cells from Human Embryos Have an Open Chromatin Configuration with Enhanced Trophoblast Differentiation Ability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Maintenance of Human Primordial Germ Cell-Like Cells in a Long-Term Culture System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Current progress onFrontiers in cell and developmental biology · 2023Review
- Prospects for fertility preservation: the ovarian organ function reconstruction techniques for oogenesis, growth and maturationFrontiers in physiology · 2023Review
- Germline stem cells in human.Signal transduction and targeted therapy · 2022Review
- Workflow Optimization for Identification of Female Germline or Oogonial Stem Cells in Human Ovarian Cortex Using Single-Cell RNA Sequence Analysis.Stem cells (Dayton, Ohio) · 2022Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primordial germ cells (PGCs) develop only into sperm and oocytes in vivo. The molecular mechanisms underlying human PGC specification are poorly understood due to inaccessibility of cell materials and lack of in vitro models for tracking the earliest stages of germ cell development. Here, we describe a defined and stepwise differentiation system for inducing pre-migratory PGC-like cells (PGCLCs) from human pluripotent stem cells (PSCs). In response to cytokines, PSCs differentiate first into a heterogeneous mesoderm-like cell population and then into PGCLCs, which exhibit minimal PRDM14 expression. PGC specification in humans is similar to the murine process, with the sequential activation of mesodermal and PGC genes, and the suppression of neural induction and of de novo DNA methylation, suggesting that human PGC formation is induced via epigenesis, the process of germ cell specification via inductive signals from surrounding somatic cells. This study demonstrates that PGC commitment in humans shares key features with that of the mouse, but also highlights key differences, including transcriptional regulation during the early stage of human PGC development (3-6 weeks). A more comprehensive understanding of human germ cell development may lead to methodology for successfully generating PSC-derived gametes for reproductive medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.